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Literature summary for 3.6.1.1 extracted from

  • Lee, M.J.; Huang, H.; Lin, W.; Yang, R.R.; Liu, C.L.; Huang, C.Y.
    Activation of Helicobacter pylori inorganic pyrophosphatase and the importance of Cys16 in thermostability, enzyme activation and quaternary structure (2007), Arch. Microbiol., 188, 473-482.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
GSH activates up to 4 mM, and inhibits at higher concentrations Helicobacter pylori
NaBH4 activates at above 12 mM Helicobacter pylori

Protein Variants

Protein Variants Comment Organism
C16S site-directed mutagenesis, the mutant shows a loss of 50% activity and a reduction in sensitivity to reductants and oxidized glutathione compared to the wild-type enzyme. In addition, the replacement causes a considerable disruption in thermostability, C16S substitution destabilizes PPase through impairing trimer-trimer interactions Helicobacter pylori
D69G site-directed mutagenesis, inactive mutant Helicobacter pylori
K103A site-directed mutagenesis, inactive mutant Helicobacter pylori
Y140F site-directed mutagenesis, the mutant shows 78% reduced activity compared to the wild-type enzyme Helicobacter pylori

Inhibitors

Inhibitors Comment Organism Structure
GSH activates up to 4 mM, and inhibits at higher concentrations Helicobacter pylori

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information effects of cysteine, homocysteine, and NaBH4 on kinetics of wild-type and mutant C16S enzymes, overview Helicobacter pylori
0.083
-
diphosphate pH 8.0, 37°C, recombinant wild-type enzyme Helicobacter pylori
0.098
-
diphosphate pH 8.0, 37°C, recombinant mutant C16S Helicobacter pylori
0.173
-
diphosphate pH 8.0, 37°C, recombinant mutant Y140F Helicobacter pylori

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Helicobacter pylori 5829
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ dependent on, other divalent cations substituted poorly for Mg2+ Helicobacter pylori
additional information no effect by Ca2+, Cd2+, and Ni2+ Helicobacter pylori

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
20000
-
6 * 20000 Helicobacter pylori

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
diphosphate + H2O Helicobacter pylori
-
2 phosphate
-
?

Organism

Organism UniProt Comment Textmining
Helicobacter pylori P56153
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
diphosphate + H2O
-
Helicobacter pylori 2 phosphate
-
?

Subunits

Subunits Comment Organism
hexamer 6 * 20000 Helicobacter pylori

Synonyms

Synonyms Comment Organism
inorganic pyrophosphatase
-
Helicobacter pylori
More Helicobacter pylori is a family I PPase Helicobacter pylori
PPase
-
Helicobacter pylori

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Helicobacter pylori

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
residue Cys116 is involved in thermostability Helicobacter pylori
37
-
15 min, the mutant enzyme loses 70% activity in absence of Mg2+, half-life is 4.2 min Helicobacter pylori
74.3
-
T1/2 of the wild-type enzyme in absence of Mg2+ Helicobacter pylori
77.6
-
T1/2 of the wild-type enzyme in presence of Mg2+ Helicobacter pylori

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
78.4
-
diphosphate pH 8.0, 37°C, recombinant mutant Y140F Helicobacter pylori
173.1
-
diphosphate pH 8.0, 37°C, recombinant mutant C16S Helicobacter pylori
343.9
-
diphosphate pH 8.0, 37°C, recombinant wild-type enzyme Helicobacter pylori

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
Helicobacter pylori

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
17.2
-
pH 8.0, 37°C, recombinant wild-type enzyme Helicobacter pylori GSH