Literature summary for 3.6.1.1 extracted from

de Graaf, B.H.; Rudd, J.J.; Wheeler, M.J.; Perry, R.M.; Bell, E.M.; Osman, K.; Franklin, F.C.; Franklin-Tong, V.E.
Self-incompatibility in Papaver targets soluble inorganic pyrophosphatases in pollen (2006), Nature, 444, 490-493.

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Cloned(Commentary)

Cloned (Comment)	Organism
expression of His-tagged enzyme	Papaver rhoeas

Inhibitors

Inhibitors	Comment	Organism	Structure
Ca2+	inhibits sPPase activity of the enzyme, 62% inhibition of p26.1a, 49% of p26.1b	Papaver rhoeas
additional information	the enzyme is inhibited by phosphorylation	Papaver rhoeas

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cytosol	-	Papaver rhoeas	5829	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	dependent on, cannot be substituted by Mn2+ or Ca2+	Papaver rhoeas

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
24400	-	x * 24400, p26.1a, SDS-PAGE, x * 26500, p26.1b, SDS-PAGE	Papaver rhoeas
26500	-	x * 24400, p26.1a, SDS-PAGE, x * 26500, p26.1b, SDS-PAGE	Papaver rhoeas

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
diphosphate + H2O	Papaver rhoeas	the enzyme is regulated by reversible phosphorylation, another mechanism in regulation of several physiological processes, e.g. self-incompatibility-mediated pollen tube inhibition, overview	2 phosphate	-	?
additional information	Papaver rhoeas	cytosolic phosphoproteins p26.1 from incompatible pollen show rapid, self-incompatibility-induced Ca2+-dependent hyperphosphorylation in vivo, overview	?	-	?

Organism

Organism	UniProt	Comment	Textmining
Papaver rhoeas	-	-	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
phosphoprotein	the enzyme is inhibited by in vitro phosphorylation, self-incompatibility induces Ca21-dependent phosphorylation of p26.1	Papaver rhoeas

Purification (Commentary)

Purification (Comment)	Organism
recombinant His-tagged enzyme by nickel affinity chromatography	Papaver rhoeas

Source Tissue

Source Tissue	Comment	Organism	Textmining
pollen	hydrated, specific for, high activity	Papaver rhoeas	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
2170	-	purified p26.1b	Papaver rhoeas
2320	-	purified p26.1a	Papaver rhoeas

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
diphosphate + H2O	-	Papaver rhoeas	2 phosphate	-	?
diphosphate + H2O	the enzyme is regulated by reversible phosphorylation, another mechanism in regulation of several physiological processes, e.g. self-incompatibility-mediated pollen tube inhibition, overview	Papaver rhoeas	2 phosphate	-	?
additional information	cytosolic phosphoproteins p26.1 from incompatible pollen show rapid, self-incompatibility-induced Ca2+-dependent hyperphosphorylation in vivo, overview	Papaver rhoeas	?	-	?

Subunits

Subunits	Comment	Organism
?	x * 24400, p26.1a, SDS-PAGE, x * 26500, p26.1b, SDS-PAGE	Papaver rhoeas

Synonyms

Synonyms	Comment	Organism
inorganic pyrophosphatase	-	Papaver rhoeas
p26.1a	-	Papaver rhoeas
p26.1b	-	Papaver rhoeas
phosphoprotein p26.1	-	Papaver rhoeas
sPPAse	-	Papaver rhoeas

pI Value

Organism	Comment	pI Value Maximum	pI Value
Papaver rhoeas	p26.1b, sequence calculation	-	6.03
Papaver rhoeas	p26.1a, sequence calculation	-	6.11

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Release 2023.1 (January 2023)