Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain BL21 pLysS(DE3) | Pyrococcus horikoshii |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Pyrococcus horikoshii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
diphosphate + H2O | Pyrococcus horikoshii | - |
2 phosphate | - |
? | |
diphosphate + H2O | Pyrococcus horikoshii DSM 12428 | - |
2 phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus horikoshii | O59570 | - |
- |
Pyrococcus horikoshii DSM 12428 | O59570 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
diphosphate + H2O | - |
Pyrococcus horikoshii | 2 phosphate | - |
? | |
diphosphate + H2O | development and evaluation of a phosphate-based colorimetric assay to measure progress of PCR using the PPase-coupled enzyme assay, overview | Pyrococcus horikoshii | 2 phosphate | - |
? | |
diphosphate + H2O | - |
Pyrococcus horikoshii DSM 12428 | 2 phosphate | - |
? | |
diphosphate + H2O | development and evaluation of a phosphate-based colorimetric assay to measure progress of PCR using the PPase-coupled enzyme assay, overview | Pyrococcus horikoshii DSM 12428 | 2 phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
inorganic pyrophosphatase | - |
Pyrococcus horikoshii |
PPase | - |
Pyrococcus horikoshii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Pyrococcus horikoshii |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | 95 | active at | Pyrococcus horikoshii |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | 95 | recombinant PhPPase, robust catalytic activity | Pyrococcus horikoshii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Pyrococcus horikoshii |