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Literature summary for 3.6.1.17 extracted from
- Isolation, purification, and characterization of P1,P4-diguanosine 5'-tetraphosphate asymmetrical-pyrophosphohydrolase from brine shrimp eggs (1965), Biochemistry, 4, 1568-1575.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | - |
Artemia salina |  |
| fluoride | - |
Artemia salina | |
| Ionic strength | significant inhibition at high ionic strength | Artemia salina | |
| p-mercuribenzoate | - |
Artemia salina | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.03 | - |
P1,P4-bis(5'-guanosyl) tetraphosphate | - |
Artemia salina | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Artemia salina | |
| Mn2+ | able to substitute for Mg2+, 70% of activity at equal metal ion concentration | Artemia salina | |
| Zn2+ | 25% of activity | Artemia salina | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| P1,P4-bis(5'-guanosyl)tetraphosphate + H2O | Artemia salina | - |
GTP + GMP | - |
ir | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Artemia salina | - |
brine shrimp | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Artemia salina |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| egg | undeveloped | Artemia salina | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.001 | - |
crude extract | Artemia salina |
| 0.2 | - |
purified enzyme | Artemia salina |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C crude salt fraction, 2-3 days | Artemia salina |
| -20°C, low ionic strength solution, several months, no loss of activity | Artemia salina |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| DNA + H2O | poor substrate | Artemia salina | ? | - |
? | |
| P1,P3-bis(5'-guanosyl) triphosphate + H2O | poor substrate | Artemia salina | ? | - |
? | |
| P1,P4-bis(5'-guanosyl)tetraphosphate + H2O | - |
Artemia salina | GTP + GMP | - |
ir | |
| RNA + H2O | poor substrate | Artemia salina | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| asym-di-GDPase | - |
- |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
- |
Artemia salina |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
5 min, complete inactivation | Artemia salina |
| 37 | - |
5 min, 18% inactivation | Artemia salina |
| 45 | - |
5 min, 69% inactivation | Artemia salina |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Artemia salina |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 10 | stable | Artemia salina |
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