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Literature summary for 3.6.1.23 extracted from

  • Bernier-Villamor, V.; Camacho, A.; Hidalgo-Zarco, F.; Perez, J.; Ruiz-Perez, L.M.; Gonzalez-Pacanowska, D.
    Characterization of deoxyuridine 5'-triphosphate nucleotidohydrolase from Trypanosoma cruzi (2002), FEBS Lett., 526, 147-150.
    View publication on PubMed
Search for more literature for 3.6.1.23

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli strain BW286 Trypanosoma cruzi

Inhibitors

Inhibitors Comment Organism Structure
5'-O-(4,4'-dimethoxytrityl)-2'-deoxyuridine
-
 Trypanosoma cruzi
alpha,beta-imido-dUDP
-
 Trypanosoma cruzi

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0005
-
 dUTP 25°C Trypanosoma cruzi
0.0062
-
 dUDP 25°C Trypanosoma cruzi

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Trypanosoma cruzi

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32000
-
 2 * 32000, SDS-PAGE, 2 * 32065, calculated from amino acid sequence Trypanosoma cruzi
32065
-
 2 * 32000, SDS-PAGE, 2 * 32065, calculated from amino acid sequence Trypanosoma cruzi
62440
-
 gel filtration Trypanosoma cruzi
64000
-
 SDS-PAGE of cross-linked subunits Trypanosoma cruzi

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
dUTP + H2O Trypanosoma cruzi removes dUTP from the nucleotide triphosphate pool and therefore prevents the incorporation of uracil into the DNA dUMP + diphosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Trypanosoma cruzi
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant protein Trypanosoma cruzi

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dUDP + H2O
-
 Trypanosoma cruzi dUMP + phosphate
-
 ?
dUTP + H2O
-
 Trypanosoma cruzi dUMP + diphosphate
-
 ?
dUTP + H2O removes dUTP from the nucleotide triphosphate pool and therefore prevents the incorporation of uracil into the DNA Trypanosoma cruzi dUMP + diphosphate
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 32000, SDS-PAGE, 2 * 32065, calculated from amino acid sequence Trypanosoma cruzi

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.8
-
 dUTP 25°C Trypanosoma cruzi

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.00024
-
 alpha,beta-imido-dUDP 25°C Trypanosoma cruzi
2.5
-
 5'-O-(4,4'-dimethoxytrityl)-2'-deoxyuridine 25°C Trypanosoma cruzi

pI Value

Organism Comment pI Value Maximum pI Value
Trypanosoma cruzi calculated from amino acid sequence
-
 4.9