Literature summary for 3.6.1.23 extracted from

Barabas, O.; Nemeth, V.; Vertessy, B.G.
Crystallization and preliminary X-ray studies of dUTPase from Mason-Pfizer monkey retrovirus (2006), Acta Crystallogr. Sect. F, F62, 399-401.

Cloned(Commentary)

Cloned (Comment)	Organism
expression as C-terminal domain fusion protein with the nucleocapsid protein in Escherichia coli	Mason-Pfizer monkey virus

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified recombinant C-terminal domain nucleocapsid fusion enzyme, hanging drop vapour diffusion method, 5 mg/ml protein in 50 mM Tris-HCl, pH 8.0, 0.2 M NH4Cl, 10 mM MgCl2, 5 mM DTT, and 0.5 mM PMSF, with or without 1.3 mM alpha,beta-imino-dUTP, room temperature, mixed 1:1 with reservoir solution containing 0.1 M Tris-HCl, pH 8.5, 8% w/v PEG 8000, X-ray diffraction structure determination and analysis at 1.75-2.3 A resolution	Mason-Pfizer monkey virus

Crystallization (Comment)

Organism

purified recombinant C-terminal domain nucleocapsid fusion enzyme, hanging drop vapour diffusion method, 5 mg/ml protein in 50 mM Tris-HCl, pH 8.0, 0.2 M NH4Cl, 10 mM MgCl2, 5 mM DTT, and 0.5 mM PMSF, with or without 1.3 mM alpha,beta-imino-dUTP, room temperature, mixed 1:1 with reservoir solution containing 0.1 M Tris-HCl, pH 8.5, 8% w/v PEG 8000, X-ray diffraction structure determination and analysis at 1.75-2.3 A resolution

Mason-Pfizer monkey virus

Organism

Organism	UniProt	Comment	Textmining
Mason-Pfizer monkey virus	-	M-PMV, a beta-retrovirus	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant C-terminal domain nucleocapsid fusion protein or free enzyme from Escherichia coli by heparin affinity chromatography and gel filtration	Mason-Pfizer monkey virus

Subunits

Subunits	Comment	Organism
trimer	homotrimer, crystal structure	Mason-Pfizer monkey virus

Synonyms

Synonyms	Comment	Organism
deoxyuridine 5'-triphosphate nucleotidohydrolase	-	Mason-Pfizer monkey virus
dUTPae	-	Mason-Pfizer monkey virus

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Release 2023.1 (January 2023)