Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.6.1.23 extracted from

  • Siggaard, J.H.; Johansson, E.; Vognsen, T.; Helt, S.S.; Harris, P.; Larsen, S.; Willemoes, M.
    Concerted bifunctionality of the dCTP deaminase-dUTPase from Methanocaldococcus jannaschii: a structural and pre-steady state kinetic analysis (2009), Arch. Biochem. Biophys., 490, 42-49.
    View publication on PubMed
Search for more literature for 3.6.1.23

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method, using 0.2 M potassium hydrogen phosphate and 20% (w/v) PEG3350 for mutant enzyme E145A complexed with alpha,beta-imido-dUTP, or 0.2 M potassium citrate and 20% (w/v) PEG3350 for mutant enzyme E145Q complexed with diphosphate Methanocaldococcus jannaschii

Protein Variants

Protein Variants Comment Organism
E145A the mutant shows reduced specific activity towards dUTP Methanocaldococcus jannaschii
E145Q the mutant shows reduced specific activity towards dUTP Methanocaldococcus jannaschii

Inhibitors

Inhibitors Comment Organism Structure
alpha,beta-imido-dUTP
-
 Methanocaldococcus jannaschii

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required for activity Methanocaldococcus jannaschii

Organism

Organism UniProt Comment Textmining
Methanocaldococcus jannaschii
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Methanocaldococcus jannaschii

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.52
-
 mutant enzyme E145Q, in 50 mM HEPES, pH 6.8, at 50°C Methanocaldococcus jannaschii
1.33
-
 mutant enzyme E145A, in 50 mM HEPES, pH 6.8, at 50°C Methanocaldococcus jannaschii
8.8
-
 wild type enzyme, in 50 mM HEPES, pH 6.8, at 50°C Methanocaldococcus jannaschii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dUTP + H2O
-
 Methanocaldococcus jannaschii dUMP + diphosphate
-
 ?

Synonyms

Synonyms Comment Organism
dCTP deaminase-dUTPase bifunctional enzyme Methanocaldococcus jannaschii