Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Dugesia ryukyuensis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.004 | - |
dUTP | in the presence of 5 mM Mg2+, at pH 8.2 and 25°C | Dugesia ryukyuensis | |
0.0073 | - |
dUTP | in the presence of 5 mM Mn2+, at pH 8.2 and 25°C | Dugesia ryukyuensis | |
0.0094 | - |
dATP | in the presence of 5 mM Mg2+, at pH 8.2 and 25°C | Dugesia ryukyuensis | |
0.077 | - |
dCTP | in the presence of 5 mM Mg2+, at pH 8.2 and 25°C | Dugesia ryukyuensis | |
0.11 | - |
dTTP | in the presence of 5 mM Mg2+, at pH 8.2 and 25°C | Dugesia ryukyuensis | |
0.21 | - |
dGTP | in the presence of 5 mM Mg2+, at pH 8.2 and 25°C | Dugesia ryukyuensis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
dUTP + H2O | Dugesia ryukyuensis | - |
dUMP + diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Dugesia ryukyuensis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography | Dugesia ryukyuensis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dATP + H2O | - |
Dugesia ryukyuensis | dAMP + diphosphate | - |
? | |
dCTP + H2O | - |
Dugesia ryukyuensis | dCMP + diphosphate | - |
? | |
dGTP + H2O | - |
Dugesia ryukyuensis | dGMP + diphosphate | - |
? | |
dTTP + H2O | - |
Dugesia ryukyuensis | dTMP + diphosphate | - |
? | |
dUTP + H2O | - |
Dugesia ryukyuensis | dUMP + diphosphate | - |
? | |
dUTP + H2O | highest activity | Dugesia ryukyuensis | dUMP + diphosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotrimer | 3 * 16554, calculated from amino acid sequence | Dugesia ryukyuensis |
Synonyms | Comment | Organism |
---|---|---|
DUT | - |
Dugesia ryukyuensis |
dUTPase | - |
Dugesia ryukyuensis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.4 | - |
dTTP | in the presence of 5 mM Mg2+, at pH 8.2 and 25°C | Dugesia ryukyuensis | |
1.5 | - |
dATP | in the presence of 5 mM Mg2+, at pH 8.2 and 25°C | Dugesia ryukyuensis | |
1.6 | - |
dCTP | in the presence of 5 mM Mg2+, at pH 8.2 and 25°C | Dugesia ryukyuensis | |
2.9 | - |
dGTP | in the presence of 5 mM Mg2+, at pH 8.2 and 25°C | Dugesia ryukyuensis | |
34 | - |
dUTP | in the presence of 5 mM Mg2+, at pH 8.2 and 25°C | Dugesia ryukyuensis | |
48 | - |
dUTP | in the presence of 5 mM Mn2+, at pH 8.2 and 25°C | Dugesia ryukyuensis |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Dugesia ryukyuensis | calculated from amino acid sequence | - |
5.7 |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
13 | - |
dTTP | in the presence of 5 mM Mg2+, at pH 8.2 and 25°C | Dugesia ryukyuensis | |
14 | - |
dGTP | in the presence of 5 mM Mg2+, at pH 8.2 and 25°C | Dugesia ryukyuensis | |
16 | - |
dATP | in the presence of 5 mM Mg2+, at pH 8.2 and 25°C | Dugesia ryukyuensis | |
21 | - |
dCTP | in the presence of 5 mM Mg2+, at pH 8.2 and 25°C | Dugesia ryukyuensis | |
6500 | - |
dUTP | in the presence of 5 mM Mn2+, at pH 8.2 and 25°C | Dugesia ryukyuensis | |
8500 | - |
dUTP | in the presence of 5 mM Mg2+, at pH 8.2 and 25°C | Dugesia ryukyuensis |