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Literature summary for 3.6.1.23 extracted from
- Potential steps in the evolution of a fused trimeric all-beta dUTPase involve a catalytically competent fused dimeric intermediate (2016), FEBS J., 283, 3268-3286 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21 (DE3) Rosetta cells | Drosophila virilis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0018 | - |
dUTP | at pH 7.5 and 20°C | Drosophila virilis |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 57900 | - |
calculated from amino acid sequence | Drosophila virilis |
| 57900 | - |
electrospray ionization mass spectrometry | Drosophila virilis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dUTP + H2O | Drosophila virilis | - |
dUMP + diphosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Drosophila virilis | B4LTG5 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA agarose affinity column chromatography and Superose 12 gel filtration | Drosophila virilis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dUTP + H2O | - |
Drosophila virilis | dUMP + diphosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| heterotrimer | dynamic light scattering | Drosophila virilis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| deoxyuridine 5'-triphosphate nucleotidohydrolase | - |
Drosophila virilis |
| DUT | - |
Drosophila virilis |
| dUTPase | - |
Drosophila virilis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | 65 | the apparent melting point of heat inactivation is around 35°C. The melting temperature by thermal denaturation is 65°C | Drosophila virilis |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 4.7 | - |
dUTP | at pH 7.5 and 20°C | Drosophila virilis | |
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Release 2023.1 (January 2023)
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