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Literature summary for 3.6.1.23 extracted from
- Structural insight into the African swine fever virus dUTPase reveals a novel folding pattern in the dUTPase family (2019), J. Virol., 94, e01698-19 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | African swine fever virus |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| in complex with dUMP and Mg2+, sitting drop vapor diffusion method, using 0.16 M sodium chloride, 0.08 M HEPES pH 7.5, 0.02 M sodium citrate tribasic dihydrate, 17% (w/v) polyethylene glycol 3350 | African swine fever virus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| D33A | the enzyme activity of the mutant is almost totally abolished | African swine fever virus |
| D91A | the enzyme activity of the mutant is almost totally abolished | African swine fever virus |
| F154A | the mutant exhibits approximately 10.5% of the activity of the wild type enzyme | African swine fever virus |
| Q120A | the enzyme activity of the mutant is almost totally abolished | African swine fever virus |
| R71A | the mutant exhibits approximately 9.6% of the activity of the wild type enzyme | African swine fever virus |
| S72A | the mutant exhibits approximately 6.5% of the activity of the wild type enzyme | African swine fever virus |
| Y94A | the mutant exhibits approximately 10% of the activity of the wild type enzyme | African swine fever virus |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | African swine fever virus |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 67000 | - |
gel filtration | African swine fever virus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dUTP + H2O | African swine fever virus | - |
dUMP + diphosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| African swine fever virus | A0A2X0SE53 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA column chromatography and Superdex S200 gel filtration | African swine fever virus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dUTP + H2O | - |
African swine fever virus | dUMP + diphosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homotrimer | - |
African swine fever virus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| deoxyuridine 5'-triphosphate pyrophosphatase | - |
African swine fever virus |
| dUTPase | - |
African swine fever virus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 84 | - |
melting temperature | African swine fever virus |
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Release 2023.1 (January 2023)
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