Literature summary for 3.6.1.23 extracted from

Li, C.; Chai, Y.; Song, H.; Weng, C.; Qi, J.; Sun, Y.; Gao, G.F.
Crystal structure of African swine fever virus dUTPase reveals a potential drug target (2019), mBio, 10, e02483-19 .

Search for more literature for 3.6.1.23

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	African swine fever virus

Crystallization (Commentary)

Crystallization (Comment)	Organism
apo state and in complex with dUMP, hanging drop vapor diffusion method, using 0.1 M ammonium citrate tribasic (pH 7.0), 12% (w/v) polyethylene glycol 3350 at 18°C (apoenzyme) or 0.1 M citric acid (pH 3.5), 14% (w/v) polyethylene glycol 1000 (enzyme in complex with dUMP)	African swine fever virus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
3.788	-	dUTP	at pH 8.0 and 37°C	African swine fever virus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
50000	-	gel filtration	African swine fever virus
50500	-	sedimentation velocity analytical ultracentrifugation	African swine fever virus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
dUTP + H2O	African swine fever virus	-	dUMP + diphosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
African swine fever virus	A0A2X0SE53	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni-NTA column chromatography and Superdex S200 gel filtration	African swine fever virus

Source Tissue

Source Tissue	Comment	Organism	Textmining

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
dUTP + H2O	-	African swine fever virus	dUMP + diphosphate	-	?
additional information	the enzyme does not hydrolyze dATP, dTTP, dCTP, or dGTP	African swine fever virus	?	-	-

Subunits

Subunits	Comment	Organism
homotrimer	3 * 17000, SDS-PAGE	African swine fever virus

Synonyms

Synonyms	Comment	Organism
DUT	-	African swine fever virus
dUTP nucleotidohydrolase	-	African swine fever virus
dUTP pyrophosphatase	-	African swine fever virus
dUTPase	-	African swine fever virus
E165R	-	African swine fever virus

General Information

General Information	Comment	Organism
physiological function	the enzyme is required for productive replication of African swine fever virus in swine macrophages	African swine fever virus

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Release 2023.1 (January 2023)