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Literature summary for 3.6.1.27 extracted from
- Purification and properties of C 55 -isoprenylpyrophosphate phosphatase from Micrococcus lysodeikticus (1972), J. Biol. Chem., 247, 5116-5122.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| ethanol | slight activation at concentration of 4 M | Micrococcus luteus |  |
| methanol | slight activation at concentration of 4 M | Micrococcus luteus | |
| additional information | significant rise in activity at high ionic strength | Micrococcus luteus | |
| n-Propanol | activating at concentrations below 1.5 M | Micrococcus luteus | |
| Triton X-100 | - |
Micrococcus luteus | |
| Triton X-114 | - |
Micrococcus luteus | |
General Stability
| General Stability | Organism |
|---|---|
| ascorbic acid stabilizes | Micrococcus luteus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| DTNB | slight inhibition | Micrococcus luteus | |
| n-butanol | - |
Micrococcus luteus | |
| n-Propanol | inhibitory at concentrations above 1.5 M | Micrococcus luteus | |
| p-chloromercuriphenylsulfonic acid | - |
Micrococcus luteus | |
| tetrahydrofuran | inhibitory at concentrations above 2 M | Micrococcus luteus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.4 | - |
undecaprenyl diphosphate | - |
Micrococcus luteus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | - |
Micrococcus luteus | 16020 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no requirement for added metal ions | Micrococcus luteus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| undecaprenyl diphosphate + H2O | Micrococcus luteus | involved in cyclic peptidoglycan pathway | undecaprenyl phosphate + phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Micrococcus luteus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Micrococcus luteus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| undecaprenyl diphosphate + H2O | - |
Micrococcus luteus | undecaprenyl phosphate + phosphate | - |
? | |
| undecaprenyl diphosphate + H2O | involved in cyclic peptidoglycan pathway | Micrococcus luteus | undecaprenyl phosphate + phosphate | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| -20 | - |
significant loss of activity by freezing and thawing one time | Micrococcus luteus |
| 4 | - |
30-40% loss of activity within 1 week | Micrococcus luteus |
| 25 | - |
solubilized enzyme stable for 30 min below 25°C, loss of activity with higher temperatures | Micrococcus luteus |
| 40 | - |
particular enzyme stable for 30 min below 40°C, loss of activity with higher temperatures | Micrococcus luteus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.3 | 7.5 | - |
Micrococcus luteus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 9 | - |
Micrococcus luteus |
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