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Literature summary for 3.6.1.42 extracted from

  • Berninsone, P.; Lin, Z.Y.; Kempner, E.; Hirschberg, C.B.
    Regulation of yeast Golgi glycosylation. Guanosine diphosphatase functions as a homodimer in the membrane (1995), J. Biol. Chem., 270, 14564-14567.
    View publication on PubMed
Search for more literature for 3.6.1.42

General Stability

General Stability Organism
stable to several cycles of freezing and thawing Saccharomyces cerevisiae

Localization

Localization Comment Organism GeneOntology No. Textmining
Golgi apparatus Golgi localization Saccharomyces cerevisiae 5794
-
Golgi apparatus distribution in multiple compartments Saccharomyces cerevisiae 5794
-
Golgi lumen
-
 Saccharomyces cerevisiae 5796
-
Golgi membrane
-
 Saccharomyces cerevisiae 139
-
membrane type II transmembrane protein with a single hydrophobic domain, which acts as an uncleaved signal sequence and membrane anchor, and is preceded by a short hydrophilic cytosolic tail Saccharomyces cerevisiae 16020
-
additional information Golgi localization Saccharomyces cerevisiae
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
additional information
-
 determination of structural and functional target sizes of GDPase activity in situ Saccharomyces cerevisiae
47000
-
 deglycosylated GDPase, result of proteolytic cleavage of the 55000 Da enzyme during purification, SDS-PAGE Saccharomyces cerevisiae
55000
-
-
 Saccharomyces cerevisiae
120000
-
 functional target size, irradiation of membranes from wild-type and gda1 null mutants Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
GDP + H2O Saccharomyces cerevisiae
-
 5'-GMP + phosphate
-
 ?
additional information Saccharomyces cerevisiae GDPase plays an important role in mannosylation of proteins and lipids by regulating the amount of GDP-mannose available in Golgi lumen ?
-
 ?
additional information Saccharomyces cerevisiae GDPase has an important role in glycosylation reactions in Golgi lumen ?
-
 ?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
 strains G2-25, wild-type and null-mutants for GDA-1
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein three potential N-glycosylation sites Saccharomyces cerevisiae

Storage Stability

Storage Stability Organism
-80°C, separated into aliquots, stable for a long time Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GDP + H2O
-
 Saccharomyces cerevisiae 5'-GMP + phosphate
-
 ?
GDP + H2O best substrate Saccharomyces cerevisiae 5'-GMP + phosphate
-
 ?
additional information GDPase plays an important role in mannosylation of proteins and lipids by regulating the amount of GDP-mannose available in Golgi lumen Saccharomyces cerevisiae ?
-
 ?
additional information GDPase has an important role in glycosylation reactions in Golgi lumen Saccharomyces cerevisiae ?
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 55000, guanosine diphosphatase functions in situ as a homodimer and does not require association with other membrane proteins for its function, immunodetection, SDS-PAGE Saccharomyces cerevisiae
More guanosine diphosphatase functions in situ as a homodimer and does not require association with other membrane proteins for its function Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.6
-
 assay at Saccharomyces cerevisiae