Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ADP-D-glucose | competitive | Escherichia coli | |
CDP-D-glucose | competitive | Escherichia coli | |
GDP-D-glucose | competitive | Escherichia coli | |
Inhibitor from E. coli | - |
Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Escherichia coli | |
0.002 | - |
UDP-D-glucose | with 5 mM CoSO4, pH 8.0, 37 °C | Escherichia coli | |
0.02 | - |
UDP-D-glucose | with 5 mM CoSO4, pH 8.0, 37 °C | Escherichia coli | |
0.11 | - |
GDP-D-glucose | - |
Escherichia coli | |
0.2 | - |
UDP-D-glucose | with 5 mM MgCl2, pH 8.0, 25 °C | Escherichia coli | |
0.33 | - |
CDP-D-glucose | - |
Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | absolute requirement for a divalent cation, at pH 8.0 in Tris-HCl buffer the requirement can be satisfied in order of decreasing affinity by Co2+, Mn2+, Mg2+. At pH 5.1 in acetate buffer only Co2+ and Mn2+ are effective | Escherichia coli | |
Mg2+ | absolute requirement for a divalent cation, at pH 8.0 in Tris-HCl buffer the requirement can be satisfied in order of decreasing affinity by Co2+, Mn2+, Mg2+. At pH 5.1 in acetate buffer only Co2+ and Mn2+ are effective | Escherichia coli | |
Mn2+ | absolute requirement for a divalent cation, at pH 8.0 in Tris-HCl buffer the requirement can be satisfied in order of decreasing affinity by Co2+, Mn2+, Mg2+. At pH 5.1 in acetate buffer only Co2+ and Mn2+ are effective | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
ATCC 12793 | - |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
12.58 | - |
- |
Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
CDP-D-glucose | at 5% of the activity with UDP-glucose | Escherichia coli | CMP + glucose 1-phosphate | - |
? | |
GDP-glucose | at 5% of the activity with UDP-glucose | Escherichia coli | GMP + glucose 1-phosphate | - |
? | |
UDP-D-glucose | - |
Escherichia coli | UMP + glucose 1-phosphate | - |
? | |
UDP-galactose | - |
Escherichia coli | UMP + galactose 1-phosphate | - |
? | |
UDP-N-acetyl-D-galactosamine | - |
Escherichia coli | UMP + N-acetyl-D-galactosamine 1-phosphate | - |
? | |
UDP-N-acetyl-D-glucosamine | - |
Escherichia coli | UMP + N-acetyl-D-glucosamine 1-phosphate | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
Co2+, Mn2+ or Mg2+ protect against heat inactivation | Escherichia coli |