Literature summary for 3.6.1.45 extracted from

Innes, D.; Beacham, I.R.; Burns, D.M.
The role of the intracellular inhibitor of periplasmic UDP-sugar hydrolase (5'-nucleotidase) in Escherichia coli: cytoplasmic localisation of 5'-nucleotidase is conditionally lethal (2001), J. Basic Microbiol., 41, 329-337.

Search for more literature for 3.6.1.45

Cloned(Commentary)

Cloned (Comment)	Organism
overexpression of wild-type and mutant UshA enzyme lacking signal peptide in Escherichia coli 5A-1, UshA lacking the signal peptide is an active cytoplasmic 5' nucleotidase which causes conditional lethality	Escherichia coli

Protein Variants

Protein Variants	Comment	Organism
additional information	carboxyterminal deletion which abolishes the 5' nucleotidase activity and deletion of the signal peptide	Escherichia coli
additional information	deletion of the signal peptide, identical to the mature periplasmic enzyme, except for the presence of an N-terminal methionine residue, expressed in Escherichia coli 5A-1	Escherichia coli

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cytoplasm	enzyme lacking signal sequence except for that encoding the initiating methionine residue, expressed in Escherichia coli 5A-1	Escherichia coli	5737	-
periplasm	wild-type expressed in Escherichia coli TG1 and 5A-1	Escherichia coli	-	-

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	-	K12 TG1 and K12 5A-1 strains	-

Synonyms

Synonyms	Comment	Organism
UshA	bifunctional enzyme with UDP-sugar hydrolase and 5' nucleotidase activity	Escherichia coli

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Release 2023.1 (January 2023)