Literature summary for 3.6.1.45 extracted from

Wang, L.; Zhou, Y.J.; Ji, D.; Lin, X.; Liu, Y.; Zhang, Y.; Liu, W.; Zhao, Z.K.
Identification of UshA as a major enzyme for NAD degradation in Escherichia coli (2014), Enzyme Microb. Technol., 58-59, 75-79.

Search for more literature for 3.6.1.45

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0012	-	AMP	pH 7.5, 30°C	Escherichia coli
0.0053	-	NAD+	pH 7.5, 30°C	Escherichia coli
0.007	-	NADH	pH 7.5, 30°C	Escherichia coli
0.0111	-	NMN	pH 7.5, 30°C	Escherichia coli

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	B1XCS0	-	-
Escherichia coli DH10B	B1XCS0	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
AMP + H2O	-	Escherichia coli	adenosine + phosphate	-	?
AMP + H2O	-	Escherichia coli DH10B	adenosine + phosphate	-	?
NAD+ + H2O	-	Escherichia coli	AMP + NMN	-	?
NAD+ + H2O	-	Escherichia coli DH10B	AMP + NMN	-	?
NADH + H2O	-	Escherichia coli	NMNH + AMP	-	?
NADH + H2O	-	Escherichia coli DH10B	NMNH + AMP	-	?
NMN + H2O	-	Escherichia coli	nicotinamide riboside + phosphate	-	?
NMN + H2O	-	Escherichia coli DH10B	nicotinamide riboside + phosphate	-	?

Synonyms

Synonyms	Comment	Organism
UshA	-	Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
10	-	NADH	pH 7.5, 30°C	Escherichia coli
19.3	-	NAD+	pH 7.5, 30°C	Escherichia coli
405	-	AMP	pH 7.5, 30°C	Escherichia coli
1492	-	NMN	pH 7.5, 30°C	Escherichia coli

General Information

General Information	Comment	Organism
physiological function	deletion of the UshA gene leads to faster cell growth and improves extracellular NAD stability by 3fold under conditions similar to whole-cell biocatalysis	Escherichia coli

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
1400	-	NADH	pH 7.5, 30°C	Escherichia coli
3700	-	NAD+	pH 7.5, 30°C	Escherichia coli
135000	-	NMN	pH 7.5, 30°C	Escherichia coli
334000	-	AMP	pH 7.5, 30°C	Escherichia coli

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Release 2023.1 (January 2023)