Any feedback?
Literature summary for 3.6.1.5 extracted from
- Purification and characteristics of functional properties of soluble nucleoside triphosphatase (apyrase) from bovine brain (2008), Biochemistry (Moscow), 73, 1047-1052.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 2-oxoglutarate | activates to 148% at 0.5 mM | Bos taurus |  |
| acetyl-CoA | activates to 148% at 0.5 mM | Bos taurus | |
| D-fructose-1,6-diphosphate | activates to 155% at 0.5 mM | Bos taurus | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ADP | 75% inhibition at 0.5 mM | Bos taurus | |
| AMP | complete inhibition at 0.5 mM | Bos taurus | |
| ATP | 68% inhibition at 0.5 mM | Bos taurus | |
| CDP | 59% inhibition at 0.5 mM | Bos taurus | |
| citrate | 20% inhibition at 0.5 mM | Bos taurus | |
| CMP | complete inhibition at 0.5 mM | Bos taurus | |
| GDP | 81% inhibition at 0.5 mM | Bos taurus | |
| GMP | complete inhibition at 0.5 mM | Bos taurus | |
| GTP | 70% inhibition at 0.5 mM | Bos taurus | |
| IDP | 59% inhibition at 0.5 mM | Bos taurus | |
| IMP | 95% inhibition at 0.5 mM | Bos taurus | |
| ITP | 42% inhibition at 0.5 mM | Bos taurus | |
| propionate | 61% inhibition at 0.5 mM | Bos taurus | |
| succinate | 55% inhibition at 0.5 mM | Bos taurus | |
| UDP | 68% inhibition at 0.5 mM | Bos taurus | |
| UMP | 92% inhibition at 0.5 mM | Bos taurus | |
| UTP | inhibitory at 0.6 mM and above, 34% inhibition at 0.5 mM | Bos taurus | |
| XTP | 60% inhibition at 0.5 mM | Bos taurus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | the enzyme shows Michaelis-Menten kinetics with all substrates except for UTP | Bos taurus |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Bos taurus | 5829 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | activates | Bos taurus | |
| Co2+ | activates | Bos taurus | |
| Cu2+ | activates | Bos taurus | |
| Mg2+ | activates, best metal ion | Bos taurus | |
| Mn2+ | activates | Bos taurus | |
| additional information | enzyme activity is absolutely dependent on divalent metal ions, Mg2+ exhibits the maximal activating effect among the studied cations. Other metal cations like Mn2+, Co2+, Ca2+, Cu2+, and Zn2+ are less efficient, and Ba2+ is not able to activate the enzyme | Bos taurus | |
| Zn2+ | activates | Bos taurus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 58600 | - |
gel filtration | Bos taurus |
| 60200 | - |
1 * 60200, SDS-PAGE | Bos taurus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme 4915fold from brain by ammonium sulfate fractionation, hydrophobic interaction chromatography, gel filtration, anion exchange and hydroxyapatite chromatography, again gel filtration, and adsorption chromatography | Bos taurus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| brain | - |
Bos taurus | - |
| erythrocyte | - |
Bos taurus | - |
| heart | - |
Bos taurus | - |
| kidney | low enzyme activity | Bos taurus | - |
| liver | high enzyme activity | Bos taurus | - |
| lung | - |
Bos taurus | - |
| skeletal muscle | - |
Bos taurus | - |
| small intestine | low enzyme activity | Bos taurus | - |
| spleen | - |
Bos taurus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
tissue-specific activity, overview | Bos taurus |
| 0.09 | - |
erythrocytes | Bos taurus |
| 0.47 | - |
liver | Bos taurus |
| 639 | - |
purifed native enzyme from brain | Bos taurus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 2 H2O | - |
Bos taurus | AMP + 2 phosphate | - |
? | |
| CDP + H2O | - |
Bos taurus | CMP + phosphate | - |
? | |
| CTP + 2 H2O | - |
Bos taurus | CMP + 2 phosphate | - |
? | |
| GTP + 2 H2O | - |
Bos taurus | GMP + 2 phosphate | - |
? | |
| ITP + 2 H2O | - |
Bos taurus | IMP + 2 phosphate | - |
? | |
| UTP + 2 H2O | - |
Bos taurus | UMP + 2 phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 60200, SDS-PAGE | Bos taurus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| NTPase | - |
Bos taurus |
| nucleoside triphosphatase | - |
Bos taurus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Bos taurus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Bos taurus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 10.5 | - |
Bos taurus |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
