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Literature summary for 3.6.1.54 extracted from

  • Cho, J.; Lee, C.; Zhao, J.; Young, H.; Zhou, P.
    Structure of the essential Haemophilus influenzae UDP-diacylglucosamine pyrophosphohydrolase LpxH in lipid A biosynthesis (2016), Nat. Microbiol., 1, 16154 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.6.1.54

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Haemophilus influenzae

Crystallization (Commentary)

Crystallization (Comment) Organism
in complex with lipid X, using 100 mM NaCl, 10 mM HEPES, 35 mM sodium acetate pH 4.1, 2.2% (w/v) PEG 4000, 0.3 mM sulfonyl piperazine, 0.7% (v/v) DMSO, 22% (v/v) glycerol, and about 3-4 mM n-decyl-beta-D-thiomaltopyranoside Haemophilus influenzae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-2-N,3-O-bis[(3R)-3-hydroxytetradecanoyl]-alpha-D-glucosamine + H2O Haemophilus influenzae
-
 2-N,3-O-bis[(3R)-3-hydroxytetradecanoyl]-alpha-D-glucosaminyl 1-phosphate + UMP
-
 ?

Organism

Organism UniProt Comment Textmining
Haemophilus influenzae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography and Sephacryl S-200 gel filtration Haemophilus influenzae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-2-N,3-O-bis[(3R)-3-hydroxytetradecanoyl]-alpha-D-glucosamine + H2O
-
 Haemophilus influenzae 2-N,3-O-bis[(3R)-3-hydroxytetradecanoyl]-alpha-D-glucosaminyl 1-phosphate + UMP
-
 ?

Synonyms

Synonyms Comment Organism
LpxH
-
 Haemophilus influenzae
UDP-diacylglucosamine pyrophosphohydrolase
-
 Haemophilus influenzae