Crystallization (Comment) | Organism |
---|---|
Ch1 | Gallus gallus |
General Stability | Organism |
---|---|
enzyme is very stable | Gallus gallus |
enzyme is very stable | Sus scrofa |
enzyme is very stable | Oryctolagus cuniculus |
enzyme is very stable | Equus caballus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetic data | Gallus gallus | |
additional information | - |
additional information | kinetic data | Sus scrofa | |
additional information | - |
additional information | kinetic data | Oryctolagus cuniculus | |
additional information | - |
additional information | kinetic data | Equus caballus | |
0.17 | - |
benzoyl phosphate | Ch2 | Gallus gallus | |
0.39 | - |
benzoyl phosphate | Ch1 monomer | Gallus gallus | |
0.48 | - |
benzoyl phosphate | Ch1 dimer | Gallus gallus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
9400 | - |
Ch1, 1 * 9400, SDS-PAGE | Gallus gallus |
9400 | - |
Ch1, 2 * 9400, SDS-PAGE | Gallus gallus |
11060 | - |
calculated from amino acid sequence | Equus caballus |
11400 | - |
Ch2, 1 * 11400, SDS-PAGE | Gallus gallus |
11900 | - |
Ch1, sedimentation equilibrium | Gallus gallus |
12000 | - |
Ch2, sedimentation equilibrium | Gallus gallus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Equus caballus | - |
- |
- |
Gallus gallus | - |
Ch1 and Ch2 are genetically specified isozymes | - |
Gallus gallus | - |
two different isozymes: Ch1 and Ch2 | - |
Oryctolagus cuniculus | - |
- |
- |
Sus scrofa | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
no modification | - |
Sus scrofa |
no modification | Ch1 and Ch2: no sugar, no hexose, no hexosamine | Gallus gallus |
Purification (Comment) | Organism |
---|---|
- |
Sus scrofa |
- |
Oryctolagus cuniculus |
- |
Equus caballus |
two isozymes: Ch1 and Ch2 | Gallus gallus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
muscle | skeletal muscle | Gallus gallus | - |
muscle | skeletal muscle | Sus scrofa | - |
muscle | skeletal muscle | Oryctolagus cuniculus | - |
muscle | skeletal muscle | Equus caballus | - |
muscle | muscle-type and common-type enzyme | Gallus gallus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
5960 | - |
Ch1 | Gallus gallus |
8500 | - |
Ch2 | Gallus gallus |
Storage Stability | Organism |
---|---|
4°C, Ch1 crystalline enzyme suspension, long-time stable | Gallus gallus |
4°C, Ch2, 70% ammonium sulfate, long-time stable | Gallus gallus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,3-diphosphoglycerate + H2O | - |
Gallus gallus | 3-phosphoglycerate + phosphate | - |
? | |
1,3-diphosphoglycerate + H2O | - |
Sus scrofa | 3-phosphoglycerate + phosphate | - |
? | |
1,3-diphosphoglycerate + H2O | - |
Oryctolagus cuniculus | 3-phosphoglycerate + phosphate | - |
? | |
1,3-diphosphoglycerate + H2O | - |
Equus caballus | 3-phosphoglycerate + phosphate | - |
? | |
acylphosphate + H2O | specifically catalyzes the hydrolysis of the carboxyl-phosphate bond of various acylphosphates | Gallus gallus | carboxylate + phosphate | - |
? | |
acylphosphate + H2O | specifically catalyzes the hydrolysis of the carboxyl-phosphate bond of various acylphosphates | Sus scrofa | carboxylate + phosphate | - |
? | |
acylphosphate + H2O | specifically catalyzes the hydrolysis of the carboxyl-phosphate bond of various acylphosphates | Oryctolagus cuniculus | carboxylate + phosphate | - |
? | |
acylphosphate + H2O | specifically catalyzes the hydrolysis of the carboxyl-phosphate bond of various acylphosphates | Equus caballus | carboxylate + phosphate | - |
? | |
benzoyl phosphate + H2O | - |
Gallus gallus | benzoate + phosphate | - |
? | |
benzoyl phosphate + H2O | - |
Sus scrofa | benzoate + phosphate | - |
? | |
benzoyl phosphate + H2O | - |
Oryctolagus cuniculus | benzoate + phosphate | - |
? | |
benzoyl phosphate + H2O | - |
Equus caballus | benzoate + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | - |
Sus scrofa |
dimer | - |
Equus caballus |
dimer | Ch1, 2 * 9400, SDS-PAGE | Gallus gallus |
monomer | - |
Sus scrofa |
monomer | - |
Oryctolagus cuniculus |
monomer | - |
Equus caballus |
monomer | Ch2, 1 * 11400, SDS-PAGE | Gallus gallus |
monomer | Ch1, 1 * 9400, SDS-PAGE | Gallus gallus |
Synonyms | Comment | Organism |
---|---|---|
Ch1 | Ch1 and Ch2 are different genetically specified isozymes | Gallus gallus |
Ch1 | multiple forms of chicken acylphosphatase, 2 isozymes: Ch1 and Ch2 differ in molecular weight, amino acid composition and kinetic parameters | Gallus gallus |
Ch2 | Ch1 and Ch2 are different genetically specified isozymes | Gallus gallus |
Ch2 | multiple forms of chicken acylphosphatase, 2 isozymes: Ch1 and Ch2 differ in molecular weight, amino acid composition and kinetic parameters | Gallus gallus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Gallus gallus |
25 | - |
assay at | Sus scrofa |
25 | - |
assay at | Oryctolagus cuniculus |
25 | - |
assay at | Equus caballus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
- |
Sus scrofa |
5 | - |
dimer of Ch1, and Ch2 | Gallus gallus |
5.3 | - |
assay at | Gallus gallus |
5.3 | - |
assay at | Sus scrofa |
5.3 | - |
assay at | Oryctolagus cuniculus |
5.3 | - |
assay at | Equus caballus |
5.3 | - |
monomer of Ch1 | Gallus gallus |