Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Urea | R97Q mutant, at low urea concentration, sharp activation with maximum, about 50% activation at 3 M urea, followed by rapid inactivation: 6 M: 80% inhibition, 8 M: 100% inhibition | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
cDNA cloning, wild-type and mutated recombinant acylphosphatase, recombinant protein expression in Escherichia coli strain BL21 | Homo sapiens |
synthetic gene coding for human muscle enzyme, expressed in Escherichia coli strain BL21 | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
DELTA1-6 deletion mutant | N-terminus truncated mutant lacks the first six residues: reduced specific activity and native-like structure | Homo sapiens |
R97Q | reduced specific activity, kinetic and structural properties of R97Q mutant indicate possible role of Arg-97 in the stabilisation of the active site correct conformation, most likely via back-bone and side chain interactions with Arg-23, the residue involved in phosphate binding by the enzyme | Homo sapiens |
Y98Q | reduced specific activity, kinetic and structural properties of Y98Q mutant indicate possible involvement of Tyr-98 in stabilisation of acylphosphatase overall structure | Homo sapiens |
General Stability | Organism |
---|---|
muscular enzyme, sensitive to urea: urea denaturation | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
phosphate | phosphate, competitive inhibition, muscular isoenzyme, wild-type, Ki: 0.98 mM, R97Q Ki: 1.79 mM, Y98Q Ki: 1.17 mM, DELTA6 deletion mutant Ki: 1.19 mM | Homo sapiens | |
Urea | reversibel inhibition, wild-type and DELTA6 deletion mutant, 4.2 M: 50% inhibition, Y98Q, 3.8 M: 50% inhibition, R97Q, at low urea concentration sharp activation, maximum activation at 3 M: 50% activation, followed by rapid inactivation, 6 M: 80% inhibition, 8 M 100% inhibition; wild-type and mutants, urea inactivation studies | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetic studies | Homo sapiens | |
0.41 | - |
benzoyl phosphate | Y98Q | Homo sapiens | |
0.51 | - |
benzoyl phosphate | wild-type, muscle | Homo sapiens | |
0.55 | - |
benzoyl phosphate | DELTA6 deletion mutant | Homo sapiens | |
1.14 | - |
benzoyl phosphate | R97Q | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
human | - |
Homo sapiens | - |
two different isoenzymes: skeletal muscle and erythrocyte enzyme | - |
Homo sapiens | - |
several mutants, wild-type, DELTA6 deletion mutant: lacking residues 1-6 at the N-terminus and mutated, R97Q and Y98Q, recombinant | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
side-chain modification | muscular isoenzyme, acetylated at the N-terminus | Homo sapiens |
Purification (Comment) | Organism |
---|---|
recombinant wild-type and mutated acylphosphatases | Homo sapiens |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
an acylphosphate + H2O = a carboxylate + phosphate | catalytic mechanism | Homo sapiens |
Renatured (Comment) | Organism |
---|---|
renaturation after treatment with urea | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
muscle | - |
Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2500 | - |
DELTA6 deletion mutant | Homo sapiens |
3600 | - |
Y98Q | Homo sapiens |
4700 | - |
R97Q | Homo sapiens |
7000 | - |
wild-type, muscle | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,3-diphosphoglycerate + H2O | - |
Homo sapiens | 3-phosphoglycerate + phosphate | - |
? | |
acetyl phosphate + H2O | - |
Homo sapiens | acetate + phosphate | - |
? | |
acylphosphate + H2O | specifically catalyzes the hydrolysis of the carboxyl-phosphate bond of various acylphosphates | Homo sapiens | carboxylate + phosphate | - |
? | |
benzoyl phosphate + H2O | - |
Homo sapiens | benzoate + phosphate | - |
? | |
carbamoyl phosphate + H2O | - |
Homo sapiens | carbamate + phosphate | - |
? | |
additional information | specifically catalyzes the hydrolysis of the carboxyl-phosphate bond of various acylphosphates | Homo sapiens | ? | - |
? | |
additional information | hydrolyzes phosphorylated intermediate formed during activity of membrane pump ATPase | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
More | two isoenzymatic forms: one prevalent in skeletal and cardiac muscle named muscle type, the other in red blood cells named erythrocyte or common type, even if both isoforms are expressed in all tissues | Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
muscle enzyme is very basic protein | Homo sapiens |
3.7 | 4.4 | R97Q | Homo sapiens |
4.7 | 5.9 | DELTA6 deletion mutant | Homo sapiens |
4.8 | 5.8 | wild-type, muscle | Homo sapiens |
5 | 5.9 | Y98Q | Homo sapiens |
5.3 | - |
assay at | Homo sapiens |