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Literature summary for 3.6.1.7 extracted from

  • Modesti, A.; Taddei, N.; Chiti, F.; Bucciantini, M.; Magherini, F.; Rigacci, S.; Stefani, M.; Raugei, G.; Ramponi, G.
    Properties of Cys21-mutated muscle acylphosphatases (1996), J. Protein Chem., 15, 27-34.
    View publication on PubMed
Search for more literature for 3.6.1.7

Cloned(Commentary)

Cloned (Comment) Organism
wild-type and Cys21 mutants: C21A and C21S, expressed in Escherichia coli strain BL21 Homo sapiens

Protein Variants

Protein Variants Comment Organism
C21A reduced specific activity, 60% compared with wild-type enzyme, kinetic and structural properties similar to those of wild-type recombinant enzyme, urea and thermal stabilities reduced: Cys21 possible involved in stabilization of enzyme active-site conformation, involved in enzyme structure stabilization, not involved in substrate binding Homo sapiens
C21S reduced specific activity, 85% compared with wild-type enzyme, kinetic and structural properties similar to those of wild-type recombinant enzyme, urea and thermal stabilities reduced: Cys21 possible involved in stabilization of enzyme active-site conformation, involved in enzyme structure stabilization, not involved in substrate binding Homo sapiens

General Stability

General Stability Organism
muscular enzyme, sensitive to urea: urea denaturation Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
Hg2+ inhibition partially reactivated in the absence of Hg2+, mutant enzymes regained 65-85% of original activity, wild-type enzyme regained less than 50% Homo sapiens
phosphate phosphate, competitive inhibition, muscular isoenzyme, wild-type, Ki: 0.9 mM, C21A Ki: 0.9 mM, C21S Ki: 0.7 mM Homo sapiens
Urea 8 M: 100% inhibition; wild-type and mutants, urea inactivation studies Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetic data Homo sapiens
0.4
-
 benzoyl phosphate C21A Homo sapiens
0.5
-
 benzoyl phosphate wild-type, muscle Homo sapiens
0.8
-
 benzoyl phosphate C21S Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
 human
-
Homo sapiens
-
 wild-type and Cys21 mutants: C21A and C21S
-

Purification (Commentary)

Purification (Comment) Organism
recombinant wild-type and Cys21 mutants: C21A and C21S Homo sapiens

Reaction

Reaction Comment Organism Reaction ID
an acylphosphate + H2O = a carboxylate + phosphate catalytic mechanism Homo sapiens
a

Renatured (Commentary)

Renatured (Comment) Organism
renaturation after treatment with urea Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
muscle
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4300
-
 C21A Homo sapiens
5900
-
 C21S Homo sapiens
7000
-
 wild-type, muscle Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,3-diphosphoglycerate + H2O
-
 Homo sapiens 3-phosphoglycerate + phosphate
-
 ?
acylphosphate + H2O specifically catalyzes the hydrolysis of the carboxyl-phosphate bond of various acylphosphates Homo sapiens carboxylate + phosphate
-
 ?
benzoyl phosphate + H2O
-
 Homo sapiens benzoate + phosphate
-
 ?
carbamoyl phosphate + H2O
-
 Homo sapiens carbamate + phosphate
-
 ?
additional information specifically catalyzes the hydrolysis of the carboxyl-phosphate bond of various acylphosphates Homo sapiens ?
-
 ?
additional information hydrolyzes the beta-aspartylphosphate formed during the action of membrane pumps Homo sapiens ?
-
 ?
succinoyl phosphate + H2O
-
 Homo sapiens succinate + phosphate
-
 ?

Synonyms

Synonyms Comment Organism
More two isoenzymatic forms: one prevalent in skeletal and cardiac muscle named muscle type, the other in red blood cells named erythrocyte or common type, even if both isoforms are expressed in all tissues Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Homo sapiens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
 thermal inactivation studies Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
additional information
-
 muscle enzyme is very basic protein Homo sapiens
4.5 5.5 C21A Homo sapiens
4.7 5.6 C21S Homo sapiens
4.8 5.8 wild-type, muscle Homo sapiens