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Literature summary for 3.6.1.9 extracted from

  • Bartkiewicz, M.; Grzybowska, E.; Sierakowska, H.; Shugar, D.
    Reassociable dimer subunit of potato nucleotide pyrophosphatase; specificity and stability (1985), Biochim. Biophys. Acta, 830, 313-319.
No PubMed abstract available
Search for more literature for 3.6.1.9

General Stability

General Stability Organism
tetrameric form stored at 4°C for longer periods remains active, but in aqueous solution higher insoluble aggregates formed Solanum tuberosum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
74000
-
 4 * 74000, SDS-PAGE Solanum tuberosum
74000
-
 2 * 74000, SDS-PAGE, alkali-treated preparation, different substrate specificity compared to the tetrameric form Solanum tuberosum
346000
-
 sucrose density gradient centrifugation Solanum tuberosum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2',3'-cAMP + H2O Solanum tuberosum low activity for the tetramer adenosine monophosphate
-
 ?
NAD+ + H2O Solanum tuberosum tetramer 4fold more active than dimer NMN + AMP
-
 ?

Organism

Organism UniProt Comment Textmining
Solanum tuberosum
-
 potato
-

Source Tissue

Source Tissue Comment Organism Textmining
tuber
-
 Solanum tuberosum
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2',3'-cAMP + H2O low activity for the tetramer Solanum tuberosum adenosine monophosphate
-
 ?
bis(p-nitrophenyl)phosphate + H2O dimer nearly 4fold more active than tetramer Solanum tuberosum p-nitrophenol + p-nitrophenylphosphate
-
 ?
NAD+ + H2O tetramer 4fold more active than dimer Solanum tuberosum NMN + AMP
-
 ?
p-nitrophenyl phenylphosphonate + H2O dimer 8fold more active than tetramer Solanum tuberosum p-nitrophenol + phenylphosphonate
-
 ?
P1,P2-di(adenosine-5') diphosphate + H2O tetramer 3fold more active than dimer Solanum tuberosum ?
-
 ?
P1,P2-di(thymidine-3') diphosphate + H2O very low activity for both dimer and tetramer Solanum tuberosum ?
-
 ?
P1,P2-di(thymidine-5') diphosphate + H2O low activity for both dimer and tetramer Solanum tuberosum ?
-
 ?
P1,P3-di(adenosine-5') triphosphate + H2O tetramer 2fold more active than dimer Solanum tuberosum ?
-
 ?
thymidine 3'-monophosphate p-nitrophenyl ester + H2O dimer 10fold more active than tetramer Solanum tuberosum thymidine 3'-monophosphate + p-nitrophenol
-
 ?
thymidine 5'-monophosphate p-nitrophenyl ester + H2O equal activity for dimer and tetramer Solanum tuberosum thymidine 5'-monophosphate + p-nitrophenol
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 74000, SDS-PAGE, alkali-treated preparation, different substrate specificity compared to the tetrameric form Solanum tuberosum
tetramer 4 * 74000, SDS-PAGE Solanum tuberosum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
 rapid loss of activity for the dimeric form, tetrameric form remains fully stable Solanum tuberosum
55
-
 full stability for the tetramer up to 1 h Solanum tuberosum