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Literature summary for 3.7.1.1 extracted from

  • Liang, X.; Liberti, D.; Li, M.; Kim, Y.T.; Hutchens, A.; Wilson, R.; Rollins, J.A.
    Oxaloacetate acetylhydrolase gene mutants of Sclerotinia sclerotiorum do not accumulate oxalic acid, but do produce limited lesions on host plants (2015), Mol. Plant Pathol., 16, 559-571.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
gene gene Ss-oah1, DNA and amino acid sequence determination and analysis, PCR-based genome walking Sclerotinia sclerotiorum

Protein Variants

Protein Variants Comment Organism
additional information generation of gene deletion DELTAss-oah1 mutants Sclerotinia sclerotiorum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
oxaloacetate + H2O Sclerotinia sclerotiorum
-
oxalate + acetate
-
?
oxaloacetate + H2O Sclerotinia sclerotiorum 1980
-
oxalate + acetate
-
?

Organism

Organism UniProt Comment Textmining
Sclerotinia sclerotiorum A7ESB3 gene Ss-oah1
-
Sclerotinia sclerotiorum 1980 A7ESB3 gene Ss-oah1
-

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
Sclerotinia sclerotiorum
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
oxaloacetate + H2O
-
Sclerotinia sclerotiorum oxalate + acetate
-
?
oxaloacetate + H2O
-
Sclerotinia sclerotiorum 1980 oxalate + acetate
-
?

Synonyms

Synonyms Comment Organism
OAH
-
Sclerotinia sclerotiorum

General Information

General Information Comment Organism
malfunction gene deletion DELTAss-oah1 mutants do not accumulate oxalate in culture or during plant infection. The defect in oxalate accumulation is fully restored on reintroduction of the wild-type Ss-oah1gene. The DELTAss-oah1 mutants are deficient in compound appressorium and sclerotium development and exhibit a severe radial growth defect on medium buffered at neutral pH. On a variety of plant hosts, the DELTAss-oah1 mutants establish very restricted lesions in which the infectious hyphae gradually lose viability. Cytological comparisons of wild-type and DELTAss-oah1 infections reveal low and no oxalate accumulation, respectively, in subcuticular hyphae. Both wild-type and mutant hyphae exhibit a transient association with viable host epidermal cells at the infection front. DELTAss-oah1 mutants exhibit significantly attenuated virulence, e.g. in infected soybean leaves. Lesions generated by the GFP-labelled DELTAss-oah1 mutants expand slowly and are delimited within 3 days Sclerotinia sclerotiorum
physiological function oxalate biogenesis in Sclerotinia. sclerotiorum appears to rely exclusively on oxaloacteate hydrolase-mediated C-C cleavage of oxaloacetate. During pathogenesis, oxalic acid accumulation is critical to the broad host range necrotrophic pathogenicity Sclerotinia sclerotiorum