Application | Comment | Organism |
---|---|---|
synthesis | L-2-haloacid dehalogenase subtype produces D-2-hydroxyalkanoic acids for chiral synthesis in industry in water and organic solvents | Pseudomonas sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1-ethyl-3-(3-dimethylaminopropyl)carbodiimide | - |
Pseudomonas sp. | |
HgSO4 | - |
Pseudomonas sp. | |
iodoacetamide | - |
Pseudomonas sp. | |
N-ethylmaleimide | - |
Pseudomonas sp. | |
p-chloromercuriphenylsulfonic acid | - |
Pseudomonas sp. | |
Woodward reagent K | - |
Pseudomonas sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.37 | - |
L-2-chloropropionate | pH 9.5, 30°C | Pseudomonas sp. | |
1.1 | - |
Monochloroacetate | pH 9.5, 30°C | Pseudomonas sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
27000 | - |
2 * 27000, SDS-PAGE | Pseudomonas sp. |
54000 | - |
gel filtration | Pseudomonas sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-chloropropionate + H2O | Pseudomonas sp. | - |
lactate + HCl | - |
? | |
L-2-haloalkanoic acid + H2O | Pseudomonas sp. | - |
D-2-hydroxyalkanoic acid + halide | - |
? | |
monobromoacetate + H2O | Pseudomonas sp. | - |
glycolate + HBr | - |
? | |
monochloroacetate + H2O | Pseudomonas sp. | - |
glycolate + HCl | - |
? | |
monoiodoacetate + H2O | Pseudomonas sp. | - |
glycolate + HI | - |
? |
Organic Solvent | Comment | Organism |
---|---|---|
n-heptane | retaining 3.3% activity of that in water with substrate 2-chloropropionate | Pseudomonas sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas sp. | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
120 | - |
- |
Pseudomonas sp. |
Storage Stability | Organism |
---|---|
-20°C, pH 7.5 + 50% glycerol, 6 months, strain YL | Pseudomonas sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-2-haloacid + H2O | - |
Pseudomonas sp. | (R)-2-hydroxyacid + halide | - |
? | |
2,2-dichloropropionate + H2O | - |
Pseudomonas sp. | propionate + HCl | - |
? | |
2-bromohexadecanoate + H2O | - |
Pseudomonas sp. | D-2-hydroxyhexadecanoate + HBr | - |
? | |
2-chloroacrylate + H2O | - |
Pseudomonas sp. | 2-hydroxyacrylate + HCl | - |
? | |
2-chloropropionate + H2O | - |
Pseudomonas sp. | lactate + HCl | - |
? | |
L-2-chloropropionate + H2O | - |
Pseudomonas sp. | D-lactate + HCl | - |
? | |
L-2-haloalkanoic acid + H2O | - |
Pseudomonas sp. | D-2-hydroxyalkanoic acid + halide | - |
? | |
monobromoacetate + H2O | - |
Pseudomonas sp. | glycolate + HBr | - |
? | |
monochloroacetate + H2O | - |
Pseudomonas sp. | glycolate + HCl | - |
? | |
monoiodoacetate + H2O | - |
Pseudomonas sp. | glycolate + HI | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 27000, SDS-PAGE | Pseudomonas sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
- |
Pseudomonas sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
enzyme retains its full activity for 30 min | Pseudomonas sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9.5 | - |
- |
Pseudomonas sp. |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4.5 | 10.5 | at 30°C for 30 min | Pseudomonas sp. |