General Stability | Organism |
---|---|
pure enzyme is completely inactive after a single freeze and thawing cycle | Pseudomonas sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5,5'-dithiobis(2-nitrobenzoate) | - |
Pseudomonas sp. | |
Ag+ | 5 mM | Pseudomonas sp. | |
Co2+ | 5 mM | Pseudomonas sp. | |
Cu2+ | 5 mM | Pseudomonas sp. | |
Fe2+ | 5 mM | Pseudomonas sp. | |
Mn2+ | 5 mM | Pseudomonas sp. | |
Ni2+ | 5 mM | Pseudomonas sp. | |
SDS | 1 mM, 80% inhibition | Pseudomonas sp. | |
Zn2+ | 5 mM | Pseudomonas sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0024 | 0.0027 | 4-chlorobenzoyl-CoA | - |
Pseudomonas sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
12000 | - |
gel filtration | Pseudomonas sp. |
31000 | - |
4 * 31000, the enzyme is active in tetrameric, octameric and higher multimeric forms, SDS-PAGE | Pseudomonas sp. |
31000 | - |
8 * 31000, the enzyme is active in tetrameric, octameric and higher multimeric forms, SDS-PAGE | Pseudomonas sp. |
31000 | - |
x * 31000, the enzyme is active in tetrameric, octameric and higher multimeric forms, SDS-PAGE | Pseudomonas sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas sp. | - |
- |
- |
Pseudomonas sp. CBS-3 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2.25 | - |
- |
Pseudomonas sp. |
Storage Stability | Organism |
---|---|
-20°C, impure preparations, stable for several months | Pseudomonas sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-bromobenzoyl-CoA + H2O | - |
Pseudomonas sp. | 4-hydroxybenzoyl-CoA + bromide | - |
? | |
4-bromobenzoyl-CoA + H2O | - |
Pseudomonas sp. CBS-3 | 4-hydroxybenzoyl-CoA + bromide | - |
? | |
4-chlorobenzoyl-CoA + H2O | - |
Pseudomonas sp. | 4-hydroxybenzoyl-CoA + chloride | - |
? | |
4-chlorobenzoyl-CoA + H2O | - |
Pseudomonas sp. CBS-3 | 4-hydroxybenzoyl-CoA + chloride | - |
? | |
4-fluorobenzoyl-CoA + H2O | no activity | Pseudomonas sp. | 4-hydroxybenzoyl-CoA + fluoride | - |
? | |
4-fluorobenzoyl-CoA + H2O | no activity | Pseudomonas sp. CBS-3 | 4-hydroxybenzoyl-CoA + fluoride | - |
? | |
4-iodobenzoyl-CoA + H2O | - |
Pseudomonas sp. | 4-hydroxybenzoyl-CoA + iodide | - |
? | |
4-iodobenzoyl-CoA + H2O | - |
Pseudomonas sp. CBS-3 | 4-hydroxybenzoyl-CoA + iodide | - |
? |
Subunits | Comment | Organism |
---|---|---|
multimer | x * 31000, the enzyme is active in tetrameric, octameric and higher multimeric forms, SDS-PAGE | Pseudomonas sp. |
octamer | 8 * 31000, the enzyme is active in tetrameric, octameric and higher multimeric forms, SDS-PAGE | Pseudomonas sp. |
tetramer | 4 * 31000, the enzyme is active in tetrameric, octameric and higher multimeric forms, SDS-PAGE | Pseudomonas sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Pseudomonas sp. |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 65 | about 60% of maximal activity at 30°C and at 65°C | Pseudomonas sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
15 min, stable | Pseudomonas sp. |
60 | - |
15 min, 30% loss of activity | Pseudomonas sp. |
65 | - |
15 min, complete loss of activity | Pseudomonas sp. |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
5 | - |
4-chlorobenzoyl-CoA | - |
Pseudomonas sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
10 | - |
- |
Pseudomonas sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
9 | 11.5 | about 50% of maximal activity at pH 9.0 and at pH 11.5 | Pseudomonas sp. |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4.5 | - |
10 min, complete loss of activity | Pseudomonas sp. |
6 | - |
60 min, 10% loss of activity | Pseudomonas sp. |
6.5 | 11 | 60 min, stable | Pseudomonas sp. |
11.5 | - |
60 min, 10% loss of activity | Pseudomonas sp. |
12 | - |
10 min, complete loss of activity | Pseudomonas sp. |