Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-chlorobenzoyl-CoA + H2O | Arthrobacter sp. | - |
4-hydroxybenzoyl-CoA + chloride | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arthrobacter sp. | - |
Arthrobacter sp. strain TM-1 | - |
Purification (Comment) | Organism |
---|---|
4-CBCoA dehalogenase is purified to homogeneity from cells of Arthrobacter sp. strain TM-1 | Arthrobacter sp. |
Renatured (Comment) | Organism |
---|---|
denaturation and renaturation experiments as functions of pH and temperature are performed | Arthrobacter sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.007 | - |
time elapsed at 45°C, 1020 min, recovery 1.7% | Arthrobacter sp. |
0.08 | - |
time elapsed at 45°C, 120 min, recovery 19.9% | Arthrobacter sp. |
0.082 | - |
time elapsed at 45°C, 0 min, recovery 20.4% | Arthrobacter sp. |
0.082 | - |
time elapsed at 45°C, 15 min, recovery 20.4% | Arthrobacter sp. |
0.082 | - |
time elapsed at 45°C, 5 min, recovery 20.4% | Arthrobacter sp. |
0.194 | - |
renaturation of 4-CBCoA dehalogenaase following exposure to elevated temperature, 55°C, activity after heating to 60°C 0.081, recovery 20.1% | Arthrobacter sp. |
0.243 | - |
renaturation of 4-CBCoA dehalogenaase following exposure to elevated temperature, 20°C, activity after heating to 60°C 0.038, recovery 9.5% | Arthrobacter sp. |
0.301 | - |
renaturation of 4-CBCoA dehalogenaase following exposure to elevated temperature, 30°C, activity after heating to 60°C 0.051, recovery 12.7% | Arthrobacter sp. |
0.349 | - |
renaturation of 4-CBCoA dehalogenaase following exposure to elevated temperature, 50°C, activity after heating to 60°C 0.082, recovery 20.4% | Arthrobacter sp. |
0.36 | - |
renaturation of 4-CBCoA dehalogenaase following exposure to elevated temperature, 40°C, activity after heating to 60°C 0.055, recovery 13.7% | Arthrobacter sp. |
0.363 | - |
renaturation of 4-CBCoA dehalogenaase after return from pH 5.2 to pH 7.4, incubation time 7 min, recovery 89% | Arthrobacter sp. |
0.365 | - |
renaturation of 4-CBCoA dehalogenaase after return from pH 10.1 to pH 7.4, incubation time 7 min, recovery 90% | Arthrobacter sp. |
0.402 | - |
renaturation of 4-CBCoA dehalogenaase following exposure to elevated temperature, 45°C, activity after heating to 60°C 0.083, recovery 20.6% | Arthrobacter sp. |
0.407 | - |
renaturation of 4-CBCoA dehalogenaase after return from pH 10.1 to pH 7.4, incubation time 17 min, recovery 99% | Arthrobacter sp. |
0.41 | - |
pH 7.4 | Arthrobacter sp. |
0.41 | - |
renaturation of 4-CBCoA dehalogenaase after return from pH 5.2 to pH 7.4, incubation time 17 min, recovery 100% | Arthrobacter sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-chlorobenzoyl-CoA + H2O | - |
Arthrobacter sp. | 4-hydroxybenzoyl-CoA + chloride | - |
? |
Subunits | Comment | Organism |
---|---|---|
trimer | - |
Arthrobacter sp. |
Synonyms | Comment | Organism |
---|---|---|
4-CBCoA dehalogenase | - |
Arthrobacter sp. |
4-chlorobenzoyl-CoA dehalogenase | - |
Arthrobacter sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
activity assay | Arthrobacter sp. |
45 | - |
activity is maximal at 45°C and is totally lost by heating to 60°C for 5 min | Arthrobacter sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
activity assay | Arthrobacter sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
when incubated for 5 Min at 1.5 mg ml-1 in buffer at 5.2 or 10.1 enzyme activity is lost completely | Arthrobacter sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
coenzyme A | - |
Arthrobacter sp. |