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Literature summary for 4.1.1.1 extracted from
- Purification, characterisation and cDNA sequencing of pyruvate decarboxylase from Zygosaccharomyces bisporus (2000), Biol. Chem., 381, 349-353.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| pyruvate | substrate activation | Zygosaccharomyces bisporus |  |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| cDNA sequence | Zygosaccharomyces bisporus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| phenylpyruvate | 10 mM, 81% inhibition | Zygosaccharomyces bisporus | |
| pyruvate | substrate inhibition above 25 mM | Zygosaccharomyces bisporus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.73 | - |
pyruvate | pH 6, 25°C, sigmoidal dependence of the reaction rate from substrate concentration, Hill coefficient 2.10 | Zygosaccharomyces bisporus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | cofactor | Zygosaccharomyces bisporus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 61000 | - |
alpha4, 4 * 61000, SDS-PAGE, 4 * 61600, amino acid sequence | Zygosaccharomyces bisporus |
| 61600 | - |
alpha4, 4 * 61000, SDS-PAGE, 4 * 61600, amino acid sequence | Zygosaccharomyces bisporus |
| 244000 | - |
active enzyme, gel filtration | Zygosaccharomyces bisporus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| pyruvate | Zygosaccharomyces bisporus | key enzyme in alcoholic fermentation | acetaldehyde + CO2 | - |
? | |
| pyruvate | Zygosaccharomyces bisporus CBS 702 | key enzyme in alcoholic fermentation | acetaldehyde + CO2 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Zygosaccharomyces bisporus | Q9UUT6 | - |
- |
| Zygosaccharomyces bisporus CBS 702 | Q9UUT6 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 41fold | Zygosaccharomyces bisporus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Zygosaccharomyces bisporus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, concentrated PDC, buffered solution containing thiamine diphosphate and MgSO4, several months, stable | Zygosaccharomyces bisporus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | nonoxidative decarboxylation of pyruvate and other 2-oxo-acids | Zygosaccharomyces bisporus | ? | - |
? | |
| additional information | nonoxidative decarboxylation of pyruvate and other 2-oxo-acids | Zygosaccharomyces bisporus CBS 702 | ? | - |
? | |
| Phenylpyruvate | - |
Zygosaccharomyces bisporus | Phenylacetaldehyde + CO2 | - |
? | |
| Phenylpyruvate | - |
Zygosaccharomyces bisporus CBS 702 | Phenylacetaldehyde + CO2 | - |
? | |
| phenylpyruvate + acetaldehyde | enzyme catalyzes a carboligation as side reaction | Zygosaccharomyces bisporus | 3-hydroxy-1-phenyl-butan-2-one + CO2 | - |
? | |
| phenylpyruvate + acetaldehyde | enzyme catalyzes a carboligation as side reaction | Zygosaccharomyces bisporus CBS 702 | 3-hydroxy-1-phenyl-butan-2-one + CO2 | - |
? | |
| pyruvate | - |
Zygosaccharomyces bisporus | acetaldehyde + CO2 | - |
? | |
| pyruvate | key enzyme in alcoholic fermentation | Zygosaccharomyces bisporus | acetaldehyde + CO2 | - |
? | |
| pyruvate | - |
Zygosaccharomyces bisporus CBS 702 | acetaldehyde + CO2 | - |
? | |
| pyruvate | key enzyme in alcoholic fermentation | Zygosaccharomyces bisporus CBS 702 | acetaldehyde + CO2 | - |
? | |
| pyruvate + phenylacetaldehyde | enzyme catalyzes a carboligation as side reaction | Zygosaccharomyces bisporus | 3-hydroxy-4-phenyl-butan-2-one + CO2 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homotetramer | alpha4, 4 * 61000, SDS-PAGE, 4 * 61600, amino acid sequence | Zygosaccharomyces bisporus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ZbPDC | - |
Zygosaccharomyces bisporus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Zygosaccharomyces bisporus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
assay at | Zygosaccharomyces bisporus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| thiamine diphosphate | - |
Zygosaccharomyces bisporus | |
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