Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 4.1.1.1 extracted from

  • Eram, M.S.; Oduaran, E.; Ma, K..
    The bifunctional pyruvate decarboxylase/pyruvate ferredoxin oxidoreductase from Thermococcus guaymasensis (2014), Archaea, 2014, 349379.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.25
-
pyruvate pH 8.4, 80°C Thermococcus guaymasensis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
12000
-
2 * 12000 + 2 * 26000 + 2 * 35000 + 2 * 46000, SDS-PAGE Thermococcus guaymasensis
26000
-
2 * 12000 + 2 * 26000 + 2 * 35000 + 2 * 46000, SDS-PAGE Thermococcus guaymasensis
35000
-
2 * 12000 + 2 * 26000 + 2 * 35000 + 2 * 46000, SDS-PAGE Thermococcus guaymasensis
46000
-
2 * 12000 + 2 * 26000 + 2 * 35000 + 2 * 46000, SDS-PAGE Thermococcus guaymasensis
258000
-
gel filtration Thermococcus guaymasensis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
pyruvate Thermococcus guaymasensis
-
acetaldehyde + CO2
-
?
pyruvate Thermococcus guaymasensis DSM 11113
-
acetaldehyde + CO2
-
?

Organism

Organism UniProt Comment Textmining
Thermococcus guaymasensis W8CQR1 and W8CQB2 and W8CQB1 and W8CR61 W8CQR1: subunit alpha, W8CQB2: subunit beta, W8CQB1: subunit gamma, W8CR61: subunit delta
-
Thermococcus guaymasensis DSM 11113 W8CQR1 and W8CQB2 and W8CQB1 and W8CR61 W8CQR1: subunit alpha, W8CQB2: subunit beta, W8CQB1: subunit gamma, W8CR61: subunit delta
-

Oxidation Stability

Oxidation Stability Organism
oxygen-sensitive enzyme, the t1/2 for the enzyme activity in 50 mM Tris-HCl, pH7.8 containing 2 mM sodium dithionite and 2 mM DTT is approximately 30 min Thermococcus guaymasensis

Purification (Commentary)

Purification (Comment) Organism
-
Thermococcus guaymasensis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.8
-
purified enzyme, pH 8.4, 80°C Thermococcus guaymasensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
pyruvate
-
Thermococcus guaymasensis acetaldehyde + CO2
-
?
pyruvate
-
Thermococcus guaymasensis DSM 11113 acetaldehyde + CO2
-
?

Subunits

Subunits Comment Organism
octamer 2 * 12000 + 2 * 26000 + 2 * 35000 + 2 * 46000, SDS-PAGE Thermococcus guaymasensis

Synonyms

Synonyms Comment Organism
bifunctional pyruvate decarboxylase/pyruvate ferredoxin oxidoreductase
-
Thermococcus guaymasensis
PDC
-
Thermococcus guaymasensis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
85
-
-
Thermococcus guaymasensis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
75 95 75°C: about 80% of maximal activity, 95°C: about 65% of maximal activity Thermococcus guaymasensis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.5
-
-
Thermococcus guaymasensis