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Literature summary for 4.1.1.1 extracted from

  • Eram, M.S.; Ma, K.
    Pyruvate decarboxylase activity of the acetohydroxyacid synthase of Thermotoga maritima (2016), Biochem. Biophys. Rep., 7, 394-399 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) Rosetta 2 cells Thermotoga maritima

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ dependent on Thermotoga maritima

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
pyruvate Thermotoga maritima
-
acetaldehyde + CO2
-
?

Organism

Organism UniProt Comment Textmining
Thermotoga maritima
-
-
-

Purification (Commentary)

Purification (Comment) Organism
DEAE column chromatography and HAP column chromatography Thermotoga maritima

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
pyruvate
-
Thermotoga maritima acetaldehyde + CO2
-
?

Synonyms

Synonyms Comment Organism
acetohydroxyacid synthase the enzyme also has approximately 10% pyruvate decarboxylase activity Thermotoga maritima
AHAS
-
Thermotoga maritima
PDC
-
Thermotoga maritima

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
85
-
-
Thermotoga maritima

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4
-
about 40% of the activity is lost after 5 days incubation at 4°C (without stirring) Thermotoga maritima

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
Thermotoga maritima

Cofactor

Cofactor Comment Organism Structure
thiamine diphosphate
-
Thermotoga maritima