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Literature summary for 4.1.1.103 extracted from
- Purification and characterization of 2,6-dihydroxybenzoate decarboxylase reversibly catalyzing nonoxidative decarboxylation (2004), Arch. Microbiol., 181, 391-397.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no activation by pyridoxal 5'-phosphate or thiamin diphosphate | Agrobacterium tumefaciens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ag+ | 1 mM, 100% loss of activity | Agrobacterium tumefaciens |  |
| Cu2+ | 1 mM, 26% loss of activity | Agrobacterium tumefaciens | |
| Hg2+ | 1 mM, 47% loss of activity | Agrobacterium tumefaciens | |
| additional information | not inhibitory: FeCl3, FeSO4, CoCl2, MgSO4, MnCl2, NiCl2, CaCl2, ZnSO4, EDTA, Tiron, 2,2?-bipyridyl, o-phenanthroline, 8-hydroxyquinoline, phenylhydrazine, hydroxylamine, iodoacetate, N-ethylmaleimide or NaBH4 | Agrobacterium tumefaciens | |
| p-chloromercuribenzoate | 1 mM, 20% loss of activity | Agrobacterium tumefaciens | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.08 | - |
2,6-dihydroxybenzoate | pH 7.0, 30°C | Agrobacterium tumefaciens | |
| 0.12 | - |
2,3-Dihydroxybenzoate | pH 7.0, 30°C | Agrobacterium tumefaciens | |
| 25.9 | - |
1,3-dihydroxybenzene | pH 7.0, 30°C | Agrobacterium tumefaciens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 38000 | - |
4 * 38000, SDS-PAGE | Agrobacterium tumefaciens |
| 138000 | - |
gel filtration | Agrobacterium tumefaciens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Agrobacterium tumefaciens | - |
- |
- |
| Agrobacterium tumefaciens IAM12048 | - |
- |
- |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4C, potassium phosphate buffer pH 7.0 containing 1 mM dithiothreitol, stable for 7 days | Agrobacterium tumefaciens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1,3-dihydroxybenzene + CO2 | - |
Agrobacterium tumefaciens | 2,6-dihydroxybenzoate | in the presence of KHCO3, the enzyme catalyzes the regioselective carboxylation of 1,3-dihydroxybenzene into 2,6-dihydroxybenzoate at a molar conversion ratio of 30% | r | |
| 1,3-dihydroxybenzene + CO2 | - |
Agrobacterium tumefaciens IAM12048 | 2,6-dihydroxybenzoate | in the presence of KHCO3, the enzyme catalyzes the regioselective carboxylation of 1,3-dihydroxybenzene into 2,6-dihydroxybenzoate at a molar conversion ratio of 30% | r | |
| 2,3-dihydroxybenzoate | 146% of the activity with 2,6-dihydroxybenzoate | Agrobacterium tumefaciens | 1,2-dihydroxybenzene + CO2 | - |
r | |
| 2,3-dihydroxybenzoate | 146% of the activity with 2,6-dihydroxybenzoate | Agrobacterium tumefaciens IAM12048 | 1,2-dihydroxybenzene + CO2 | - |
r | |
| 2,6-dihydroxybenzoate | - |
Agrobacterium tumefaciens | 1,3-dihydroxybenzene + CO2 | - |
r | |
| 2,6-dihydroxybenzoate | - |
Agrobacterium tumefaciens IAM12048 | 1,3-dihydroxybenzene + CO2 | - |
r | |
| additional information | no substrates: 2,4-dihydroxybenzoate, 4-hydroxybenzoate, 3-hydroxybenzoate, 2-hydroxybenzoate, 4-methoxybenzoate, 4-aminobenzoate, 2,4,6-trihydroxybenzoate and 3,4,5-trihydroxybenzoate, benzoate and terephthalate | Agrobacterium tumefaciens | ? | - |
? | |
| additional information | no substrates: 2,4-dihydroxybenzoate, 4-hydroxybenzoate, 3-hydroxybenzoate, 2-hydroxybenzoate, 4-methoxybenzoate, 4-aminobenzoate, 2,4,6-trihydroxybenzoate and 3,4,5-trihydroxybenzoate, benzoate and terephthalate | Agrobacterium tumefaciens IAM12048 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 38000, SDS-PAGE | Agrobacterium tumefaciens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
- |
Agrobacterium tumefaciens |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
30 min, 18% loss of activity | Agrobacterium tumefaciens |
| 70 | - |
30 min, complete loss of activity | Agrobacterium tumefaciens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
- |
Agrobacterium tumefaciens |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 10 | more than 80% of maximum activtiy within | Agrobacterium tumefaciens |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no cofactor required | Agrobacterium tumefaciens |
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