Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | the enzyme does not require activation by an endogenous PanM-like protein, incontrast to other species like Escherichia coli or Salmonella enterica | Corynebacterium glutamicum | |
YhhK | or PanM, the protein interacts directly with PanD, such interactions accelerate pro-PanD maturation, analysis by yeast two hybrid system. One PanM monomer (14.5 kDa) interacts with one PanD tetramer (55.6 kDa) | Salmonella enterica |
Cloned (Comment) | Organism |
---|---|
gene panD, expression of Corynebacterium glutamicum panD in Salmonella enterica panM deficient strain JE13233 shows functional complementation, thus the Corynebacterium glutamicum enzyme does not required panM activation | Salmonella enterica |
gene panD, heterologous expression in panM-deficient Salmonella enterica strain JE13153, functional complementation in PanD activity | Corynebacterium glutamicum |
Protein Variants | Comment | Organism |
---|---|---|
additional information | the PanM-deficient Salmonella enterica strain JE13153 is inactive due to impaired activation of PanD | Salmonella enterica |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
55600 | - |
- |
Salmonella enterica |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-aspartate | Corynebacterium glutamicum | - |
beta-alanine + CO2 | - |
? | |
L-aspartate | Salmonella enterica | - |
beta-alanine + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Corynebacterium glutamicum | - |
gene panD | - |
Salmonella enterica | - |
gene panD | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | the enzyme PanD is synthesized as pro-PanD, which undergoes an auto-proteolytic cleavage at residue Ser25 to yield the catalytic pyruvoyl moiety of the enzyme, interaction with YhhK, i.e. PanM, accelerates the maturation process | Salmonella enterica |
Purification (Comment) | Organism |
---|---|
native enzyme from Salmonella enterica strain JE13153 lacking panM by anion exchange chromatography | Salmonella enterica |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-aspartate | - |
Corynebacterium glutamicum | beta-alanine + CO2 | - |
? | |
L-aspartate | - |
Salmonella enterica | beta-alanine + CO2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | - |
Salmonella enterica |
Synonyms | Comment | Organism |
---|---|---|
aspartate decarboxylase | - |
Corynebacterium glutamicum |
aspartate decarboxylase | - |
Salmonella enterica |
L-Aspartate-alpha-decarboxylase | - |
Corynebacterium glutamicum |
L-Aspartate-alpha-decarboxylase | - |
Salmonella enterica |
PanD | - |
Corynebacterium glutamicum |
PanD | - |
Salmonella enterica |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyruvoyl cofactor | the enzyme PanD is synthesized as pro-PanD, which undergoes an auto-proteolytic cleavage at residue Ser25 to yield the catalytic pyruvoyl moiety of the enzyme | Corynebacterium glutamicum | |
pyruvoyl cofactor | the enzyme PanD is synthesized as pro-PanD, which undergoes an auto-proteolytic cleavage at residue Ser25 to yield the catalytic pyruvoyl moiety of the enzyme | Salmonella enterica |
General Information | Comment | Organism |
---|---|---|
additional information | the enzyme PanD is synthesized as pro-PanD, which undergoes an auto-proteolytic cleavage at residue Ser25 to yield the catalytic pyruvoyl moiety of the enzyme | Salmonella enterica |