Cloned (Comment) | Organism |
---|---|
gene panD, recombinant expression of the enzyme in Escherichia coli strain BL21(DE3) | Corynebacterium glutamicum |
Protein Variants | Comment | Organism |
---|---|---|
additional information | synthesis of beta-alanine from L-aspartate using L-aspartate-alpha-decarboxylase from Corynebacterium glutamicum recombinantly expressed in Escherichia coli strain BL21(DE3). A pH-stat directed, fed-batch feeding strategy is developed for enzymatic synthesis of beta-alanine to keep the pH value within pH 6.0-7.2 and attenuate substrate inhibition. A maximum conversion of 97.2% is obtained with an initial 5 g L-aspartate/l and another three feedings of 0.5 % w/v L-aspartate at 8 h intervals. The final beta-alanine concentration is 12.85 g/l after 36 h, method optimization, overview. Recombinant enzyme ADC shows best activity in sodium phosphate buffer at pH 6 and more than 80% activity remained between pH 4-7. The Escherichia coli BL21 (DE3)-pET heterologous system provides higher expression efficiency compared with that of homologous expression, which results in a 24fold increase in specific activity of crude enzyme | Corynebacterium glutamicum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-aspartate | Corynebacterium glutamicum | - |
beta-alanine + CO2 | - |
? | |
L-aspartate | Corynebacterium glutamicum ATCC 13032 | - |
beta-alanine + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Corynebacterium glutamicum | Q9X4N0 | - |
- |
Corynebacterium glutamicum ATCC 13032 | Q9X4N0 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from Escherichia coli strain BL21(DE3) by anion exchange chromatography, ultrafiltration, and gel filtration | Corynebacterium glutamicum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
103 | - |
purified recombinant enzyme, pH 6.0, 37°C | Corynebacterium glutamicum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-aspartate | - |
Corynebacterium glutamicum | beta-alanine + CO2 | - |
? | |
L-aspartate | - |
Corynebacterium glutamicum ATCC 13032 | beta-alanine + CO2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ADC | - |
Corynebacterium glutamicum |
ADCC.g | - |
Corynebacterium glutamicum |
L-Aspartate-alpha-decarboxylase | - |
Corynebacterium glutamicum |
PanD | - |
Corynebacterium glutamicum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
recombinant enzyme | Corynebacterium glutamicum |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 85 | activity range, profile overview | Corynebacterium glutamicum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
16 | 37 | purified enzyme, completely stable within this range, pH 6.0, after 12 h | Corynebacterium glutamicum |
55 | - |
purified enzyme, 62% activity remaining, pH 6.0, after 12 h | Corynebacterium glutamicum |
70 | - |
purified enzyme, complete loss of activity, pH 6.0, after 12 h | Corynebacterium glutamicum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
recombinant enzyme | Corynebacterium glutamicum |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 9 | activity range, profile overview | Corynebacterium glutamicum |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3 | 8 | purified enzyme, 37°C, over 60% activity remaining within this range, after 12 h | Corynebacterium glutamicum |
4 | 7 | purified enzyme, 37°C, over 80% activity remaining within this range, after 12 h | Corynebacterium glutamicum |