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Literature summary for 4.1.1.112 extracted from
- Oxalacetate decarboxylase and pyruvate carboxylase activities, and effect of sulfhydryl reagents in malic enzyme from Sulfolobus solfataricus (1988), Biochim. Biophys. Acta, 957, 301-311.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 5,5'-dithiobis(2-nitrobenzoate) | - |
Saccharolobus solfataricus |  |
| citrate | - |
Saccharolobus solfataricus | |
| EDTA | - |
Saccharolobus solfataricus | |
| glycine-NaOH | - |
Saccharolobus solfataricus | |
| N-ethylmaleimide | - |
Saccharolobus solfataricus | |
| p-hydroxymercuribenzoate | partly prevented by NADP+ | Saccharolobus solfataricus | |
| phosphate | - |
Saccharolobus solfataricus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.7 | - |
oxaloacetate | - |
Saccharolobus solfataricus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mn2+ | required, can be substituted by Mg2+, Ca2+ | Saccharolobus solfataricus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 49000 | - |
2 * 49000, SDS-PAGE | Saccharolobus solfataricus |
| 105000 | - |
gel filtration | Saccharolobus solfataricus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| oxaloacetate | Saccharolobus solfataricus | - |
? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharolobus solfataricus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Saccharolobus solfataricus |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| oxaloacetate = pyruvate + CO2 | pyruvate + CO2 = oxaloacetae | Saccharolobus solfataricus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 2.3 | - |
- |
Saccharolobus solfataricus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Oxaloacetate | - |
Saccharolobus solfataricus | Pyruvate + CO2 | - |
? | |
| oxaloacetate | - |
Saccharolobus solfataricus | ? | - |
? | |
| Pyruvate + CO2 | - |
Saccharolobus solfataricus | Oxaloacetate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 49000, SDS-PAGE | Saccharolobus solfataricus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 85 | - |
- |
Saccharolobus solfataricus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 85 | - |
half life for inacivation, 5 h | Saccharolobus solfataricus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.6 | - |
- |
Saccharolobus solfataricus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | 9 | - |
Saccharolobus solfataricus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | one pyridine nucleotide reduced for each pyruvate formed | Saccharolobus solfataricus | |
| NADP+ | one pyridine nucleotide reduced for each pyruvate formed | Saccharolobus solfataricus | |
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