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Literature summary for 4.1.1.112 extracted from
- Role of conserved residues within helices IV and VIII of the oxaloacetate decarboxylase beta subunit in the energy coupling mechanism of the Na+ pump (2002), Eur. J. Biochem., 269, 2997-3004.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli | Klebsiella pneumoniae |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| N373D | beta subunit, no protection against tryptic digestion by Na+ | Klebsiella pneumoniae |
| N373L | beta subunit, very low activity, no protection against tryptic digestion by Na+ | Klebsiella pneumoniae |
| R389A | beta subunit, significantly increased pH-optimum | Klebsiella pneumoniae |
| R389D | beta subunit, very low activity | Klebsiella pneumoniae |
| S382A | beta subunit, no activity below 20 mM Na+, active with 400 mM NaCl, no protection against tryptic digestion by Na+, no Na+ transport | Klebsiella pneumoniae |
| S382D | beta subunit, very low activity | Klebsiella pneumoniae |
| S382T | beta subunit, very low activity | Klebsiella pneumoniae |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| NaCl | almost complete inhibition at 0.4 M | Klebsiella pneumoniae |  |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | beta and gamma subunits are integral membrane proteins, alpha subunit is attached to the gamma subunit | Klebsiella pneumoniae | 16020 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Na+ | protects against tryptic digestion | Klebsiella pneumoniae | |
| Zn2+ | binds to the gamma subunit | Klebsiella pneumoniae | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Oxaloacetate | Klebsiella pneumoniae | two Na+ are transported into the periplasm and one H+ is transported into the cytoplasm during the reaction | Pyruvate + CO2 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Klebsiella pneumoniae | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme from Escherichia coli | Klebsiella pneumoniae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Oxaloacetate | - |
Klebsiella pneumoniae | Pyruvate + CO2 | - |
? | |
| Oxaloacetate | two Na+ are transported into the periplasm and one H+ is transported into the cytoplasm during the reaction | Klebsiella pneumoniae | Pyruvate + CO2 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| trimer | alpha, beta, gamma | Klebsiella pneumoniae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.25 | 6.75 | wild type enzyme | Klebsiella pneumoniae |
| 7 | - |
pH optimum at about pH 7 for all mutant enzymes except R389A | Klebsiella pneumoniae |
| 9.2 | - |
R389A mutant | Klebsiella pneumoniae |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| biotin | binds to the C-terminal domain of the alpha-subunit | Klebsiella pneumoniae | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | Ki-values given for inhibition with NaCl for all mutant enzymes at 3-4 different pH | Klebsiella pneumoniae | |
| 198 | - |
NaCl | wild type enzyme, pH 6.9 | Klebsiella pneumoniae | |
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Release 2023.1 (January 2023)
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