Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 4.1.1.12 extracted from

  • El-Rahmany, T.A.
    Comparison of L-aspartate 4-carboxy-lyases of Cunninghammela elegans and Penicillium citrinum (1994), Microbiol. Res., 149, 253-157.
    View publication on PubMed
Search for more literature for 4.1.1.12

Activating Compound

Activating Compound Comment Organism Structure
2-oxoglutarate activates Cunninghamella elegans
2-oxoglutarate activates Penicillium citrinum
oxalacetate
-
 Cunninghamella elegans
oxalacetate
-
 Penicillium citrinum
pyruvate activates Cunninghamella elegans
pyruvate activates Penicillium citrinum

Inhibitors

Inhibitors Comment Organism Structure
Cu2+
-
 Cunninghamella elegans
Cu2+
-
 Penicillium citrinum
EDTA
-
 Cunninghamella elegans
EDTA
-
 Penicillium citrinum
iodoacetate
-
 Cunninghamella elegans
iodoacetate
-
 Penicillium citrinum
Zn2+
-
 Cunninghamella elegans
Zn2+
-
 Penicillium citrinum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
25
-
 L-Asp
-
 Cunninghamella elegans
27
-
 L-Asp
-
 Penicillium citrinum

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ stimulates Cunninghamella elegans
Co2+ stimulates Penicillium citrinum
Fe2+ stimulates Cunninghamella elegans
Fe2+ stimulates Penicillium citrinum
Mn2+ stimulates Cunninghamella elegans
Mn2+ stimulates Penicillium citrinum
Ni2+ stimulates Cunninghamella elegans
Ni2+ stimulates Penicillium citrinum

Organism

Organism UniProt Comment Textmining
Cunninghamella elegans
-
-
-
Penicillium citrinum
-
-
-

Storage Stability

Storage Stability Organism
-15°C, 0.05 M acetate buffer, pH 5.2, 52% loss of activity after 6 days, complete loss of activity after 16 days Cunninghamella elegans
-15°C, 0.05 M acetate buffer, pH 5.2, 59% loss of activity after 6 days, complete loss of activity after 12 days Penicillium citrinum
4°C, 0.05 M acetate buffer, pH 5.2, rapid loss of activity Cunninghamella elegans
4°C, 0.05 M acetate buffer, pH 5.2, rapid loss of activity Penicillium citrinum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-Asp highly specific for L-Asp Cunninghamella elegans L-Ala + CO2
-
 ?
L-Asp highly specific for L-Asp Penicillium citrinum L-Ala + CO2
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Cunninghamella elegans
40
-
-
 Penicillium citrinum

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 50 30°C: about 65% of maximal activity, 50°C: about 50% of maximal activity Cunninghamella elegans
30 50 30°C: about 60% of maximal activity, 50°C: about 35% of maximal activity Penicillium citrinum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 30% loss of activity after 15 min, 75% loss of activity after 40 min Cunninghamella elegans
50
-
 37% loss of activity after 15 min, 85% loss of activity after 40 min Penicillium citrinum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
-
 Cunninghamella elegans
5.5
-
-
 Penicillium citrinum

pH Range

pH Minimum pH Maximum Comment Organism
4 8 pH 4.0: about 65% of maximal activity, pH 8.0: about 40% of maximal activity Cunninghamella elegans
4 8 pH 4.0: about 60% of maximal activity, pH 8.0: about 35% of maximal activity Penicillium citrinum

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate activates Cunninghamella elegans
pyridoxal 5'-phosphate activates Penicillium citrinum