Protein Variants | Comment | Organism |
---|---|---|
additional information | expression and characterization of naturally occurring shorter forms of enzyme. Isoform GAD67 with N-terminal deletion of 70 or 90 amino acids has about 20% of wild-type activity | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.5 | - |
L-glutamate | mutant with N-terminal deletion of 90 amino acids, pH 7.2 | Homo sapiens | |
0.69 | - |
L-glutamate | mutant with N-terminal deletion of 70 amino acids, pH 7.2 | Homo sapiens | |
0.71 | - |
L-glutamate | wild-type, pH 7.2 | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
recombinant isoform GAD67 | - |
Sus scrofa | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | both full length enzyme and a shorter form with aminoterminal deletion | Sus scrofa | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.039 | - |
mutant with N-terminal deletion of 70 amino acids, pH 7.2 | Homo sapiens |
0.041 | - |
mutant with N-terminal deletion of 90 amino acids, pH 7.2 | Homo sapiens |
0.216 | - |
wild-type, pH 7.2 | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamate | - |
Homo sapiens | 4-aminobutanoate + CO2 | - |
? | |
L-glutamate | - |
Sus scrofa | 4-aminobutanoate + CO2 | - |
? |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Sus scrofa | |
pyridoxal 5'-phosphate | Km-value 74 nM, wild-type, 490 nM, mutant with N-terminal deletion of 70 amino acids | Homo sapiens |