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Literature summary for 4.1.1.15 extracted from

  • Hiraga, K.; Ueno, Y.; Oda, K.
    Glutamate decarboxylase from Lactobacillus brevis: activation by ammonium sulfate (2008), Biosci. Biotechnol. Biochem., 72, 1299-1306.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
ammonium sulfate at 1.8 M Levilactobacillus brevis
additional information sodium glutamate is essential for tetramer formation and its activation Levilactobacillus brevis

Cloned(Commentary)

Cloned (Comment) Organism
gene gadB, DNA and amin acid sequence determination and analysis, expression of the His-tagged enzyme in Escherichia coli strains Rosetta-gami B (DE3) ad JM109 Levilactobacillus brevis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1
-
L-glutamate pH 4.6, 37°C, native enzyme Levilactobacillus brevis
1.4
-
L-glutamate pH 4.6, 37°C, recombinant enzyme Levilactobacillus brevis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
54000
-
2 * 54000, inactive, recombinant His-tagged enzyme, SDS-PAGE Levilactobacillus brevis
54000
-
4 * 54000, ammonium sulfate-activated, recombinant His-tagged enzyme, SDS-PAGE Levilactobacillus brevis
110000
-
recombinant enzyme, pH 7.0, gel filtration Levilactobacillus brevis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-glutamate Levilactobacillus brevis
-
4-aminobutanoate + CO2
-
?
L-glutamate Levilactobacillus brevis IFO 12005
-
4-aminobutanoate + CO2
-
?

Organism

Organism UniProt Comment Textmining
Levilactobacillus brevis A9ZM78 gene gadB
-
Levilactobacillus brevis IFO 12005 A9ZM78 gene gadB
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli strain Rosetta-gami B (DE3) by nickel affinity chromatography Levilactobacillus brevis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-glutamate
-
Levilactobacillus brevis 4-aminobutanoate + CO2
-
?
L-glutamate
-
Levilactobacillus brevis IFO 12005 4-aminobutanoate + CO2
-
?

Subunits

Subunits Comment Organism
dimer 2 * 54000, inactive, recombinant His-tagged enzyme, SDS-PAGE Levilactobacillus brevis
More sodium glutamate is essential for tetramer formation and its activation Levilactobacillus brevis
tetramer 4 * 54000, ammonium sulfate-activated, recombinant His-tagged enzyme, SDS-PAGE Levilactobacillus brevis

Synonyms

Synonyms Comment Organism
GAD
-
Levilactobacillus brevis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
native enzyme Levilactobacillus brevis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2 4.6 native enzyme Levilactobacillus brevis
4.5 5 recombinant His-tagged enzyme Levilactobacillus brevis

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
Levilactobacillus brevis