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Literature summary for 4.1.1.15 extracted from

  • Zhao, A.; Hu, X.; Li, Y.; Chen, C.; Wang, X.
    Extracellular expression of glutamate decarboxylase B in Escherichia coli to improve gamma-aminobutyric acid production (2016), AMB Express, 6, 55 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.1.1.15

Cloned(Commentary)

Cloned (Comment) Organism
gene gabB, recombinant expression in Escherichia coli strain BL21(DE3), intracellular and extracellular expression of GadB from BL21(DE3)/pET20b-gadB, BL21(DE3)/pET20b-pelBgadB, and BL21(DE3)/pET20b-torA-gadB, subcloning in Escherichia coli strain JM109 Escherichia coli

Protein Variants

Protein Variants Comment Organism
additional information production method optimization, overexpression of Escherichia coli gene gadB in strain BL21(DE3), overview. Some GadB proteins overexpressed in BL21(DE3)/pET20b-pelB-gadB might not be functional. The highest extracellular GadB activity (1.39 U/mL) is found in BL21(DE3)/pET20b-torAgadB with 0.7 mM IPTG induction Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli P69910
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-
Escherichia coli K-12 / W3110 P69910
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-

Synonyms

Synonyms Comment Organism
GadB
-
 Escherichia coli
glutamate decarboxylase B
-
 Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Escherichia coli

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37
-
 recombinant enzyme in Escherichia coli cells, pH 4.6, loss of 50% activity after 3 h, of 70% activity after 12 h, and of 80% activity after 36 h Escherichia coli
50
-
 recombinant enzyme in Escherichia coli cells, pH 4.6, loss of about 70% activity after 3 h, of over 80% activity after 12 h, and of over 90% activity after 36 h Escherichia coli
100
-
 purified recombinant enzyme inactivation after 10 min boiling Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.6
-
-
 Escherichia coli