Cloned (Comment) | Organism |
---|---|
gene gadlbhye1, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3) | Levilactobacillus brevis |
Protein Variants | Comment | Organism |
---|---|---|
additional information | the production of gamma-aminobutyric acid in Escherichia coli BL21 harboring gadlbhye1/pET28a is increased by adding pyridoxine as a cheaper coenzyme | Levilactobacillus brevis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4.99 | - |
L-glutamate | pH 4.2, 45°C, recombinant enzyme | Levilactobacillus brevis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamate | Levilactobacillus brevis | - |
4-aminobutanoate + CO2 | - |
ir | |
L-glutamate | Levilactobacillus brevis HYE1 | - |
4-aminobutanoate + CO2 | - |
ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Levilactobacillus brevis | A0A0B5CSE1 | isolated from Korean traditional fermented food, kimchi | - |
Levilactobacillus brevis HYE1 | A0A0B5CSE1 | isolated from Korean traditional fermented food, kimchi | - |
Purification (Comment) | Organism |
---|---|
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Levilactobacillus brevis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamate | - |
Levilactobacillus brevis | 4-aminobutanoate + CO2 | - |
ir | |
L-glutamate | - |
Levilactobacillus brevis HYE1 | 4-aminobutanoate + CO2 | - |
ir |
Subunits | Comment | Organism |
---|---|---|
? | x * 54000, recombinant His-tagged enzyme, SDS-PAGE | Levilactobacillus brevis |
Synonyms | Comment | Organism |
---|---|---|
GadB | - |
Levilactobacillus brevis |
gadlbhye1 | - |
Levilactobacillus brevis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
- |
Levilactobacillus brevis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
75.18 | - |
Tm of purified recombinant His-tagged enzyme | Levilactobacillus brevis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4 | - |
- |
Levilactobacillus brevis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Levilactobacillus brevis |
General Information | Comment | Organism |
---|---|---|
evolution | the amino acid sequences of GADLbHYE1 shows 48% homology with the GadA family and 99% identity with the GadB family from Lactobacillus brevis | Levilactobacillus brevis |
metabolism | glutamate decarboxylase is a key enzyme that catalyzes the irreversible alpha-decarboxylation of L-glutamate to 4-aminobutanoate, GABA | Levilactobacillus brevis |