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Literature summary for 4.1.1.15 extracted from

  • Lim, H.S.; Seo, D.H.; Cha, I.T.; Lee, H.; Nam, Y.D.; Seo, M.J.
    Expression and characterization of glutamate decarboxylase from Lactobacillus brevis HYE1 isolated from kimchi (2018), World J. Microbiol. Biotechnol., 34, 44 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene gadlbhye1, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3) Levilactobacillus brevis

Protein Variants

Protein Variants Comment Organism
additional information the production of gamma-aminobutyric acid in Escherichia coli BL21 harboring gadlbhye1/pET28a is increased by adding pyridoxine as a cheaper coenzyme Levilactobacillus brevis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.99
-
L-glutamate pH 4.2, 45°C, recombinant enzyme Levilactobacillus brevis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-glutamate Levilactobacillus brevis
-
4-aminobutanoate + CO2
-
ir
L-glutamate Levilactobacillus brevis HYE1
-
4-aminobutanoate + CO2
-
ir

Organism

Organism UniProt Comment Textmining
Levilactobacillus brevis A0A0B5CSE1 isolated from Korean traditional fermented food, kimchi
-
Levilactobacillus brevis HYE1 A0A0B5CSE1 isolated from Korean traditional fermented food, kimchi
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Levilactobacillus brevis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-glutamate
-
Levilactobacillus brevis 4-aminobutanoate + CO2
-
ir
L-glutamate
-
Levilactobacillus brevis HYE1 4-aminobutanoate + CO2
-
ir

Subunits

Subunits Comment Organism
? x * 54000, recombinant His-tagged enzyme, SDS-PAGE Levilactobacillus brevis

Synonyms

Synonyms Comment Organism
GadB
-
Levilactobacillus brevis
gadlbhye1
-
Levilactobacillus brevis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
-
Levilactobacillus brevis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
75.18
-
Tm of purified recombinant His-tagged enzyme Levilactobacillus brevis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4
-
-
Levilactobacillus brevis

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
Levilactobacillus brevis

General Information

General Information Comment Organism
evolution the amino acid sequences of GADLbHYE1 shows 48% homology with the GadA family and 99% identity with the GadB family from Lactobacillus brevis Levilactobacillus brevis
metabolism glutamate decarboxylase is a key enzyme that catalyzes the irreversible alpha-decarboxylation of L-glutamate to 4-aminobutanoate, GABA Levilactobacillus brevis