Cloned (Comment) | Organism |
---|---|
gene speA, recombinant expression of His6-tagged enzyme in Escherichia coli strain SM10 | Pseudomonas aeruginosa |
Protein Variants | Comment | Organism |
---|---|---|
additional information | the PA14 aguA-Gm, DELTAagu2ABCA' mutant cannot break down agmatine. Some clinical isolates of Pseudomonas aeruginosa also lack a functional agmatine deiminase | Pseudomonas aeruginosa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-arginine | Pseudomonas aeruginosa | - |
agmatine + CO2 | - |
? | |
L-arginine | Pseudomonas aeruginosa UCBPP-PA14 | - |
agmatine + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | A0A0H2ZHZ8 | - |
- |
Pseudomonas aeruginosa UCBPP-PA14 | A0A0H2ZHZ8 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His6-tagged enzyme from Escherichia coli strain SM10 by nickel affinity chromatography | Pseudomonas aeruginosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-arginine | - |
Pseudomonas aeruginosa | agmatine + CO2 | - |
? | |
L-arginine | - |
Pseudomonas aeruginosa UCBPP-PA14 | agmatine + CO2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 70600, about, recombinant His6-tagged enzyme, sequence calculation | Pseudomonas aeruginosa |
Synonyms | Comment | Organism |
---|---|---|
ADC | - |
Pseudomonas aeruginosa |
SpeA | - |
Pseudomonas aeruginosa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Pseudomonas aeruginosa |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
purified recombinant enzyme, pH 8.4, 10 min, inactivation | Pseudomonas aeruginosa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.4 | - |
assay at | Pseudomonas aeruginosa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Pseudomonas aeruginosa |
General Information | Comment | Organism |
---|---|---|
additional information | analysis of agatine content in clinical isolates PA002. PA004, PA005, PA006, and PA016, and PA14 wild-type strain, as well as the PA14 aguA-Gm, DELTAagu2ABCA' mutant strain, which lacks a functional agmatine deiminase | Pseudomonas aeruginosa |