Cloned (Comment) | Organism |
---|---|
gene adiA, recombinant expression of C-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3) | Salmonella enterica subsp. enterica serovar Typhimurium |
Crystallization (Comment) | Organism |
---|---|
purified recombinant His6-tagged apoenzyme without cofactor, microbatch method, mixing of 0.002 ml of 10 mg/ml protein in 25 mM Tris, pH 8.0, and 50 mM NaCl, with 0.002 ml of crystallization solution containing 2.0 M NaCl, 10% PEG 8000, and 25 mM Tris, pH 8.0, and placing under a layer of paraffin and silicone oils (1:1), 20°C, X-ray diffraction structure determination and analysis at 3.1 A resolution, molecular replacement using a polyalanine chain of the Escherichia coli enzyme, PDB ID 2VYC, as search model | Salmonella enterica subsp. enterica serovar Typhimurium |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
800000 | - |
about, recombinant enzyme, crystal structure | Salmonella enterica subsp. enterica serovar Typhimurium |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-arginine | Salmonella enterica subsp. enterica serovar Typhimurium | - |
agmatine + CO2 | - |
? | |
L-arginine | Salmonella enterica subsp. enterica serovar Typhimurium 4/74 | - |
agmatine + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Salmonella enterica subsp. enterica serovar Typhimurium | E8X9U7 | - |
- |
Salmonella enterica subsp. enterica serovar Typhimurium 4/74 | E8X9U7 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant C-terminally His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, dialysis, and ultrafiltration, to homogeneity | Salmonella enterica subsp. enterica serovar Typhimurium |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-arginine | - |
Salmonella enterica subsp. enterica serovar Typhimurium | agmatine + CO2 | - |
? | |
L-arginine | - |
Salmonella enterica subsp. enterica serovar Typhimurium 4/74 | agmatine + CO2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
decamer | (alphabeta)5, 10 * 80000, about, recombinant His6-tagged enzyme, SDS-PAGE | Salmonella enterica subsp. enterica serovar Typhimurium |
More | a pentamer of dimers, structure of dimer and decamer, overview. unlike the interactions between 5fold related protomers, interactions that stabilize the dimeric structure are not pH-dependent | Salmonella enterica subsp. enterica serovar Typhimurium |
Synonyms | Comment | Organism |
---|---|---|
ADC | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
AdiA | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
StADC | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | PLP, the cofactor binding at the active site induces conformational changes in the enzyme, pyridoxal 5'-phosphate binding is important for substrate binding, the substrate might bind to ADC only after PLP binding | Salmonella enterica subsp. enterica serovar Typhimurium |
General Information | Comment | Organism |
---|---|---|
additional information | the cofactor binding at the active site induces conformational changes in the enzyme, pyridoxal 5'-phosphate binding is important for substrate binding, the substrate might bind to ADC only after pyridoxal 5'-phosphate binding | Salmonella enterica subsp. enterica serovar Typhimurium |