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Literature summary for 4.1.1.19 extracted from

  • Deka, G.; Bharath, S.R.; Savithri, H.S.; Murthy, M.R.N.
    Structural studies on the decameric S. typhimurium arginine decarboxylase (ADC) Pyridoxal 5-phosphate binding induces conformational changes (2017), Biochem. Biophys. Res. Commun., 490, 1362-1368 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene adiA, recombinant expression of C-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3) Salmonella enterica subsp. enterica serovar Typhimurium

Crystallization (Commentary)

Crystallization (Comment) Organism
purified recombinant His6-tagged apoenzyme without cofactor, microbatch method, mixing of 0.002 ml of 10 mg/ml protein in 25 mM Tris, pH 8.0, and 50 mM NaCl, with 0.002 ml of crystallization solution containing 2.0 M NaCl, 10% PEG 8000, and 25 mM Tris, pH 8.0, and placing under a layer of paraffin and silicone oils (1:1), 20°C, X-ray diffraction structure determination and analysis at 3.1 A resolution, molecular replacement using a polyalanine chain of the Escherichia coli enzyme, PDB ID 2VYC, as search model Salmonella enterica subsp. enterica serovar Typhimurium

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
800000
-
about, recombinant enzyme, crystal structure Salmonella enterica subsp. enterica serovar Typhimurium

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-arginine Salmonella enterica subsp. enterica serovar Typhimurium
-
agmatine + CO2
-
?
L-arginine Salmonella enterica subsp. enterica serovar Typhimurium 4/74
-
agmatine + CO2
-
?

Organism

Organism UniProt Comment Textmining
Salmonella enterica subsp. enterica serovar Typhimurium E8X9U7
-
-
Salmonella enterica subsp. enterica serovar Typhimurium 4/74 E8X9U7
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant C-terminally His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, dialysis, and ultrafiltration, to homogeneity Salmonella enterica subsp. enterica serovar Typhimurium

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-arginine
-
Salmonella enterica subsp. enterica serovar Typhimurium agmatine + CO2
-
?
L-arginine
-
Salmonella enterica subsp. enterica serovar Typhimurium 4/74 agmatine + CO2
-
?

Subunits

Subunits Comment Organism
decamer (alphabeta)5, 10 * 80000, about, recombinant His6-tagged enzyme, SDS-PAGE Salmonella enterica subsp. enterica serovar Typhimurium
More a pentamer of dimers, structure of dimer and decamer, overview. unlike the interactions between 5fold related protomers, interactions that stabilize the dimeric structure are not pH-dependent Salmonella enterica subsp. enterica serovar Typhimurium

Synonyms

Synonyms Comment Organism
ADC
-
Salmonella enterica subsp. enterica serovar Typhimurium
AdiA
-
Salmonella enterica subsp. enterica serovar Typhimurium
StADC
-
Salmonella enterica subsp. enterica serovar Typhimurium

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate PLP, the cofactor binding at the active site induces conformational changes in the enzyme, pyridoxal 5'-phosphate binding is important for substrate binding, the substrate might bind to ADC only after PLP binding Salmonella enterica subsp. enterica serovar Typhimurium

General Information

General Information Comment Organism
additional information the cofactor binding at the active site induces conformational changes in the enzyme, pyridoxal 5'-phosphate binding is important for substrate binding, the substrate might bind to ADC only after pyridoxal 5'-phosphate binding Salmonella enterica subsp. enterica serovar Typhimurium