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Literature summary for 4.1.1.28 extracted from

  • Jin, C.M.; Yang, Y.J.; Huang, H.S.; Lim, S.C.; Kai, M.; Lee, M.K.
    Induction of dopamine biosynthesis by l-DOPA in PC12 cells: implications of L-DOPA influx and cyclic AMP (2008), Eur. J. Pharmacol., 591, 88-95.
    View publication on PubMed
Search for more literature for 4.1.1.28

Activating Compound

Activating Compound Comment Organism Structure
L-Dopa L-DOPA treatment (20-200 microM) increases the levels of dopamine by 226%-504% after 3-6 h of treatment and enhances the activities of tyrosine hydroxylase and aromatic L-amino acid decarboxylase Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
Benserazide levels of intracellular dopamine after L-DOPA treatment (0.02 and 0.1 mM) are significantly decreased by the AADC inhibitor benserazide (0.02 mM) for 6-24 h exposure prior to L-DOPA treatment in PC12 cells, the 230%-350% increases in dopamine levels by L-DOPA are reduced to 187%-284% by benserazide for 6 h Rattus norvegicus
carbidopa levels of intracellular dopamine after L-DOPA treatment (0.02 and 0.1 mM) are significantly decreased by the AADC inhibitor carbidopa (0.020 mM) for 6-24 h exposure prior to L-DOPA treatment in PC12 cells, the 230%-350% increases in dopamine levels by L-DOPA are reduced to 187%-284% by 153%-248% by carbidopa for 6 h Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3,4-Dihydroxyphenylalanine Rattus norvegicus L-DOPA, substrate exerts apoptotic cytotoxicity towards PC12 cells at a concentration of 0.1-0.2 mM for 24-48 h Dopamine + CO2 major amine neurotransmitter ?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
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-
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Source Tissue

Source Tissue Comment Organism Textmining
PC-12 cell derived from a pheochromocytoma of the rat adrenal medulla Rattus norvegicus
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Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
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 110% activity compared to control level, treatment with 0.02 mM L-DOPA in PC-12 cells at 1 h (time point) Rattus norvegicus
additional information
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 237% activity compared to control level, treatment with 0.02 mM L-DOPA in PC-12 cells at 3 h (time point) Rattus norvegicus
additional information
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 242% activity compared to control level, treatment with 0.1 mM L-DOPA in PC-12 cells at 1 h (time point) Rattus norvegicus
additional information
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 269% activity compared to control level, treatment with f 0.1 mM L-DOPA in PC-12 cells at 3 h (time point) Rattus norvegicus
additional information
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 311% activity compared to control level, treatment with 0.2 mM L-DOPA in PC-12 cells at 1 h (time point) Rattus norvegicus
additional information
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 348% activity compared to control level, treatment with 0.2 mM L-DOPA in PC-12 cells at 3 h (time point) Rattus norvegicus
additional information
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 The activity of AADC begins to decrease to about 177% (0.02 mM L-DOPA), 233% (0.1 mM L-DOPA) and 297% (0.2 mM L-DOPA) of the control levels by 3-6 h, and then returns to control level by about 24-48 h Rattus norvegicus
0.0325
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 activity of AADC, control level without treatment of L-DOPA in PC-12 cells Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3,4-Dihydroxyphenylalanine L-DOPA, substrate exerts apoptotic cytotoxicity towards PC12 cells at a concentration of 0.1-0.2 mM for 24-48 h Rattus norvegicus Dopamine + CO2 major amine neurotransmitter ?

Synonyms

Synonyms Comment Organism
AADC
-
 Rattus norvegicus
Aromatic L-amino acid decarboxylase
-
 Rattus norvegicus