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Literature summary for 4.1.1.28 extracted from
- Aromatic L-amino acid decarboxylase phosphorylation and activation by PKGIalpha in vitro (2010), J. Neurochem., 114, 542-552.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| cGMPdependent protein kinase Ialpha | phosphorylates and activates neuronal AAAD, maximal increase of activity is obtained at about 100-175 units during a 10 min incubation at 30°C | Mus musculus |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli expression vector pFLAG-1 | Mus musculus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.034 | - |
L-Dopa | in 50 mM sodium phosphate buffer, pH 7.2, 0.1 mM pargyline, 0.17 mM ascorbic acid, 0.1 mM EDTA, 1 mM beta-mercaptoethanol, 0.01 mMM pyridoxal 5'-phosphate and 0.5 mM L-Dopa, at 30°C | Mus musculus |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 50000 | - |
x * 50000, about 50000 Da, SDS-PAGE | Mus musculus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mus musculus | O88533 | - |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| phosphoprotein | - |
Mus musculus |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| either protein A-agarose column chromatography or anti-FLAG M2 affinity gel filtration | Mus musculus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| brain | - |
Mus musculus | - |
| corpus striatum | - |
Mus musculus | - |
| neuron | - |
Mus musculus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-Dopa | - |
Mus musculus | dopamine + CO2 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 50000, about 50000 Da, SDS-PAGE | Mus musculus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AAAC | - |
Mus musculus |
| Aromatic L-amino acid decarboxylase | - |
Mus musculus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| pyridoxal 5'-phosphate | - |
Mus musculus | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | neuronal AAAD plays a role in the biosynthesis of catecholamines, indoleamines and trace amines in the nervous system | Mus musculus |
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