Activating Compound | Comment | Organism | Structure |
---|---|---|---|
cGMPdependent protein kinase Ialpha | phosphorylates and activates neuronal AAAD, maximal increase of activity is obtained at about 100-175 units during a 10 min incubation at 30°C | Mus musculus |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli expression vector pFLAG-1 | Mus musculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.034 | - |
L-Dopa | in 50 mM sodium phosphate buffer, pH 7.2, 0.1 mM pargyline, 0.17 mM ascorbic acid, 0.1 mM EDTA, 1 mM beta-mercaptoethanol, 0.01 mMM pyridoxal 5'-phosphate and 0.5 mM L-Dopa, at 30°C | Mus musculus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
x * 50000, about 50000 Da, SDS-PAGE | Mus musculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | O88533 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | - |
Mus musculus |
Purification (Comment) | Organism |
---|---|
either protein A-agarose column chromatography or anti-FLAG M2 affinity gel filtration | Mus musculus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Mus musculus | - |
corpus striatum | - |
Mus musculus | - |
neuron | - |
Mus musculus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-Dopa | - |
Mus musculus | dopamine + CO2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 50000, about 50000 Da, SDS-PAGE | Mus musculus |
Synonyms | Comment | Organism |
---|---|---|
AAAC | - |
Mus musculus |
Aromatic L-amino acid decarboxylase | - |
Mus musculus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Mus musculus |
General Information | Comment | Organism |
---|---|---|
physiological function | neuronal AAAD plays a role in the biosynthesis of catecholamines, indoleamines and trace amines in the nervous system | Mus musculus |