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Literature summary for 4.1.1.31 extracted from
- CoASAc-independent phosphoenolpyruvate carboxylase from an obligate methylotroph Hyphomicrobium methylovorum GM2 - purification and characterization (1995), Biosci. Biotechnol. Biochem., 59, 140-142.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ag+ | - |
Hyphomicrobium methylovorum |  |
| Asp | - |
Hyphomicrobium methylovorum | |
| ATP | - |
Hyphomicrobium methylovorum | |
| Cd2+ | - |
Hyphomicrobium methylovorum | |
| citrate | - |
Hyphomicrobium methylovorum | |
| Cu2+ | - |
Hyphomicrobium methylovorum | |
| Fe2+ | - |
Hyphomicrobium methylovorum | |
| glycerate | - |
Hyphomicrobium methylovorum | |
| glyoxylate | - |
Hyphomicrobium methylovorum | |
| malate | - |
Hyphomicrobium methylovorum | |
| Ni2+ | - |
Hyphomicrobium methylovorum | |
| PCMB | - |
Hyphomicrobium methylovorum | |
| succinate | - |
Hyphomicrobium methylovorum | |
| Zn2+ | - |
Hyphomicrobium methylovorum | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | 18% of the activation with Mg2+ | Hyphomicrobium methylovorum | |
| Fe2+ | 12% of the activation with Mg2+ | Hyphomicrobium methylovorum | |
| Mg2+ | divalent cation required, Mg2+ is the best activator | Hyphomicrobium methylovorum | |
| Mn2+ | 23% of the activation with Mg2+ | Hyphomicrobium methylovorum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 88000 | - |
4 * 88000, SDS-PAGE | Hyphomicrobium methylovorum |
| 389000 | - |
gel filtration | Hyphomicrobium methylovorum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Hyphomicrobium methylovorum | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Hyphomicrobium methylovorum |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 104 | - |
- |
Hyphomicrobium methylovorum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| phosphoenolpyruvate + CO2 | - |
Hyphomicrobium methylovorum | phosphate + oxaloacetate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 88000, SDS-PAGE | Hyphomicrobium methylovorum |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
30 min, pH 7, no loss of activity | Hyphomicrobium methylovorum |
| 30 | - |
30 min, pH 7, 15% loss of activity | Hyphomicrobium methylovorum |
| 35 | - |
30 min, 40% loss of activity | Hyphomicrobium methylovorum |
| 40 | - |
30 min, pH 7, 89% loss of activity | Hyphomicrobium methylovorum |
| 45 | - |
30 min, pH 7, complete loss of activity | Hyphomicrobium methylovorum |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| acetyl-CoA | no effect | Hyphomicrobium methylovorum | |
| NADPH | activates | Hyphomicrobium methylovorum | |
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