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Literature summary for 4.1.1.31 extracted from
- Molecular characterization of a phosphoenolpyruvate carboxylase from a thermophilic cyanobacterium, Synechococcus vulcanus with unusual allosteric properties (2002), Plant Cell Physiol., 43, 159-169.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 3-phosphoglycerate | slight stimulation | Thermostichus vulcanus |  |
| fructose 1,6-diphosphate | activates | Thermostichus vulcanus | |
| Gln | slight stimulation | Thermostichus vulcanus | |
| Glu | slight stimulation | Thermostichus vulcanus | |
| glucose 1-phosphate | slight stimulation | Thermostichus vulcanus | |
| glucose 6-phosphate | slight stimulation | Thermostichus vulcanus | |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Thermostichus vulcanus |
General Stability
| General Stability | Organism |
|---|---|
| retains full activity after exposure to 0.5 M guanidine-HCl at 30°C for 20 h | Thermostichus vulcanus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| alpha-ketoglutarate | 10 mM, 9% inhibition | Thermostichus vulcanus | |
| ATP | 5.0 mM, 57% inhibition | Thermostichus vulcanus | |
| D-fructose | 10 mM, 19% inhibition | Thermostichus vulcanus | |
| D-Fructose 1-phosphate | 10 mM, 26% inhibition | Thermostichus vulcanus | |
| D-fructose 2,6-diphosphate | 0.3 mM, 35% inhibition | Thermostichus vulcanus | |
| fumarate | 10 mM, 31% inhibition | Thermostichus vulcanus | |
| L-Asp | more sensitive to inhibition by Asp at pH 9.0 than at pH 7.0 | Thermostichus vulcanus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 120000 | - |
4 * 120000, SDS-PAGE | Thermostichus vulcanus |
| 570000 | - |
gel filtration | Thermostichus vulcanus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermostichus vulcanus | P0A3X6 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Thermostichus vulcanus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| phosphoenolpyruvate + CO2 | - |
Thermostichus vulcanus | phosphate + oxaloacetate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 120000, SDS-PAGE | Thermostichus vulcanus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| SvPEPC | - |
Thermostichus vulcanus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 42 | - |
at pH 7.5 | Thermostichus vulcanus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 50 | 30°C: about 40% of maximal activity, 50°C: about 55% of maximal activity | Thermostichus vulcanus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
60 min, stable | Thermostichus vulcanus |
| 60 | - |
10 min, 50% loss of activity | Thermostichus vulcanus |
| 70 | - |
20 min, complete inactivation | Thermostichus vulcanus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9 | - |
at 30°C | Thermostichus vulcanus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 9.5 | pH 7.0: about 65% of maximal activity, pH 9.5: about 80% of maximal activity | Thermostichus vulcanus |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.025 | - |
Asp | pH 9.0 | Thermostichus vulcanus | |
| 2 | - |
Asp | pH 7.5 | Thermostichus vulcanus | |
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