Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
111000 | - |
4 * 111000 Determined by: 1D native-PAGE and 2D BN-/SDS-PAGE, combined with immunoblotting | Chlamydomonas reinhardtii |
669000 | - |
x * 669000, class-2 PEPC enzyme-forms a high-molecular-mass, hetero-oligomeric complex containing both CrPpc1 (p109) and CrPpc2 (p131) polypeptides. Determined by: 1D nativePAGE and 2D BN/SDSPAGE, combined with immunoblotting | Chlamydomonas reinhardtii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Chlamydomonas reinhardtii | - |
- |
- |
Purification (Comment) | Organism |
---|---|
isoform-specific purification of CrPpc1 and CrPpc2 is carried out by affinity-purification of using CrPpc1 and CrPpc2 peptide antibodies | Chlamydomonas reinhardtii |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Chlamydomonas reinhardtii | - |
additional information | CrPpc1/2 polypeptide levels are up-regulated as the initial supply of NH4Cl decreases from 10 to 0.5 mM. However, within 5 h after re-supply of 10 mM NH4Cl to the N-deficient cells, the CrPpc1/2 levels reverts back nearly to those observed in high-N grown cells | Chlamydomonas reinhardtii | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.029 | - |
with 7.5 mM NH4Cl | Chlamydomonas reinhardtii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphoenolpyruvate + CO2 | - |
Chlamydomonas reinhardtii | phosphate + oxaloacetate | - |
? |
Subunits | Comment | Organism |
---|---|---|
heteromer | x * 669000, class-2 PEPC enzyme-forms a high-molecular-mass, hetero-oligomeric complex containing both CrPpc1 (p109) and CrPpc2 (p131) polypeptides. Determined by: 1D nativePAGE and 2D BN/SDSPAGE, combined with immunoblotting | Chlamydomonas reinhardtii |
homotetramer | 4 * 111000 Determined by: 1D native-PAGE and 2D BN-/SDS-PAGE, combined with immunoblotting | Chlamydomonas reinhardtii |
Synonyms | Comment | Organism |
---|---|---|
Class-1 PEPC | - |
Chlamydomonas reinhardtii |
Class-2 PEPC | - |
Chlamydomonas reinhardtii |
CrPpc1 | - |
Chlamydomonas reinhardtii |
CrPpc2 | - |
Chlamydomonas reinhardtii |
PEP-carboxylase | - |
Chlamydomonas reinhardtii |
PEPC | - |
Chlamydomonas reinhardtii |
PPC | - |
Chlamydomonas reinhardtii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Chlamydomonas reinhardtii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.4 | - |
assay at | Chlamydomonas reinhardtii |