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Literature summary for 4.1.1.31 extracted from

  • Cousins, A.B.; Baroli, I.; Badger, M.R.; Ivakov, A.; Lea, P.J.; Leegood, R.C.; von Caemmerer, S.
    The role of phosphoenolpyruvate carboxylase during C4 photosynthetic isotope exchange and stomatal conductance (2007), Plant Physiol., 145, 1006-1017.
    View publication on PubMedView publication on EuropePMC

Protein Variants

Protein Variants Comment Organism
additional information heterozygous (Pp) and homozygous (pp) forms of a PEPC-deficient mutant of the C4 dicot Amaranthus edulis are analysed Amaranthus edulis
additional information rates of CO2 assimilation in air drop to 78% and 10% of the wild-type values in heterozygous and homozygous mutants, respectively. Stomatal conductance in air (531 microbar CO2) is similar in the wild-type and heterozygous mutant but the homozygous mutant has only 41% of the wild-type steady-state conductance under white light and the stomata opens more slowly in response to increased light or reduced CO2 partial pressure, suggesting that the C4 PEPC isoform plays an essential role in stomatal opening. Little difference in delta13C between the heterozygous mutant and wild type, indicating that leakiness, the ratio of CO2 leak rate out of the bundle sheath to the rate of CO2 supply by the C4 cycle, a measure of the coordination of C4 photosynthesis, is not affected by a 60% reduction in PEPC activity Amaranthus edulis

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
Amaranthus edulis

Organism

Organism UniProt Comment Textmining
Amaranthus edulis
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
Amaranthus edulis
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
PEPC activity is reduced to 42% and 3% of wild type activity in heterozygous and homozygous mutant plants, respectively Amaranthus edulis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
phosphoenolpyruvate + CO2
-
Amaranthus edulis phosphate + oxaloacetate
-
?

Synonyms

Synonyms Comment Organism
PEPC
-
Amaranthus edulis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Amaranthus edulis