Inhibitors | Comment | Organism | Structure |
---|---|---|---|
GMP | competitive with GDP | Lithobates catesbeianus | |
iodoacetamide | guanine nucleotides, IDP and Mn2+ protect | Lithobates catesbeianus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Lithobates catesbeianus | 5739 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | absolutely required for phosphoenolpyruvate carboxylation | Lithobates catesbeianus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
65000 | - |
gel filtration | Lithobates catesbeianus |
70000 | - |
1 * 70000, SDS-PAGE | Lithobates catesbeianus |
71000 | - |
glycerol density gradient centrifugation | Lithobates catesbeianus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lithobates catesbeianus | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Lithobates catesbeianus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
17.7 | - |
- |
Lithobates catesbeianus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GTP + oxaloacetate | - |
Lithobates catesbeianus | GDP + phosphoenolpyruvate + CO2 | - |
? | |
additional information | ADP is completely inactive in carboxylation of oxaloacetate | Lithobates catesbeianus | ? | - |
? | |
UDP + phosphoenolpyruvate + CO2 | very low activity | Lithobates catesbeianus | UTP + oxaloacetate | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 70000, SDS-PAGE | Lithobates catesbeianus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.2 | - |
phosphoenolpyruvate carboxylation and exchange reaction in presence of Mn2+ | Lithobates catesbeianus |
7.5 | - |
oxaloacetate decarboxylation in presence of Mn2+ | Lithobates catesbeianus |
7.5 | - |
oxaloacetate decarboxylation and exchange reaction in presence of Mg2+ | Lithobates catesbeianus |