Literature summary for 4.1.1.32 extracted from

Jabalquinto, A.M.; Laivenieks, M.; Cabezas, M.; Zeikus, J.G.; Cardemil, E.
The effect of active site mutations in the oxaloacetate decarboxylase and pyruvate kinase-like activities of Anaerobiospirillum succiniciproducens phosphoenolpyruvate carboxykinase (2002), J. Protein Chem., 21, 443-445.

Search for more literature for 4.1.1.32

Protein Variants

Protein Variants	Comment	Organism
D262N	active site mutant, 5times higher catalytic efficiency than wild-type enzyme	Anaerobiospirillum succiniciproducens
D263N	increased Km-value for Mn2+ and phosphoenolpyruvate	Anaerobiospirillum succiniciproducens
H225Q	active site mutant, increased Km-value for Mn2+ and phosphoenolpyruvate	Anaerobiospirillum succiniciproducens
T249N	active site mutant	Anaerobiospirillum succiniciproducens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.28	-	oxaloacetate	pH 7.5, 30°C, D262N mutant	Anaerobiospirillum succiniciproducens
0.42	-	oxaloacetate	pH 7.5, 30°C, wild-type enzyme	Anaerobiospirillum succiniciproducens
1.3	-	oxaloacetate	pH 7.5, 30°C, T249N mutant	Anaerobiospirillum succiniciproducens
2.5	-	oxaloacetate	pH 7.5, 30°C, above, D263N and H225Q mutants	Anaerobiospirillum succiniciproducens

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mn2+	enzyme-bound, role of His-225 and Asp-263 in Mn2+ binding, Asp-262 and Thr-249 are also part of the metal binding sites	Anaerobiospirillum succiniciproducens

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
GTP + oxaloacetate	Anaerobiospirillum succiniciproducens	decarboxylation of oxaloacetate is the physiological important reaction	GDP + phosphoenolpyruvate + CO2	-	?

Organism

Organism	UniProt	Comment	Textmining
Anaerobiospirillum succiniciproducens	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant wild-type and mutant enzyme	Anaerobiospirillum succiniciproducens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
additional information	-	-	Anaerobiospirillum succiniciproducens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
GTP + oxaloacetate	role of His-225 and Asp-263 in phosphoenolpyruvate binding	Anaerobiospirillum succiniciproducens	GDP + phosphoenolpyruvate + CO2	-	r
GTP + oxaloacetate	decarboxylation of oxaloacetate is the physiological important reaction	Anaerobiospirillum succiniciproducens	GDP + phosphoenolpyruvate + CO2	-	?

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
30	-	assay at	Anaerobiospirillum succiniciproducens

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	-	assay at	Anaerobiospirillum succiniciproducens

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Release 2023.1 (January 2023)