Literature summary for 4.1.1.32 extracted from

Hidalgo, J.; Latorre, P.; Carrodeguas, J.A.; Velazquez-Campoy, A.; Sancho, J.; Lopez-Buesa, P.
Inhibition of pig phosphoenolpyruvate carboxykinase isoenzymes by 3-mercaptopicolinic acid and novel inhibitors (2016), PLoS ONE, 11, e0159002 .

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Protein Variants

Protein Variants	Comment	Organism
M139L	natural variant, leading to different kinetic properties	Sus scrofa

Inhibitors

Inhibitors	Comment	Organism	Structure
3-Mercaptopicolinic acid	-	Sus scrofa
N'1-([5-[1-methyl-5-(trifluoromethyl)-1H-pyrazol-3-yl]-2-thienyl]methylidene)-2,4-dichlorobenzene-1-carbohydrazide	compound exhibits significantly different inhibitory effects on wild-type and natural variant M139L, it enhances threefold the apparent Km value for GTP in wild-type, and reduces Km value for M139L from 99 to 69 microM	Sus scrofa

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cytosol	-	Sus scrofa	5829	-

Organism

Organism	UniProt	Comment	Textmining
Sus scrofa	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
GDP + phosphoenolpyruvate + CO2	-	Sus scrofa	GTP + oxaloacetate	-	r
GTP + oxaloacetate	-	Sus scrofa	GDP + phosphoenolpyruvate + CO2	-	r

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.016	-	3-Mercaptopicolinic acid	wild-type, substrate phosphoenolpyruvate, pH 7.4, temperature not specified in the publication	Sus scrofa
0.018	-	3-Mercaptopicolinic acid	wild-type, substrate oxaloacetate, pH 7.4, temperature not specified in the publication	Sus scrofa
0.019	-	3-Mercaptopicolinic acid	mutant M139L, substrate phosphoenolpyruvate, pH 7.4, temperature not specified in the publication	Sus scrofa
0.029	-	3-Mercaptopicolinic acid	mutant M139L, substrate oxaloacetate, pH 7.4, temperature not specified in the publication	Sus scrofa
0.045	-	3-Mercaptopicolinic acid	mutant M139L, substrate GDP, pH 7.4, temperature not specified in the publication	Sus scrofa
0.045	-	3-Mercaptopicolinic acid	wild-type, substrate GDP, pH 7.4, temperature not specified in the publication	Sus scrofa
0.273	-	3-Mercaptopicolinic acid	wild-type, substrate GTP, pH 7.4, temperature not specified in the publication	Sus scrofa
0.873	-	3-Mercaptopicolinic acid	mutant M139L, substrate GTP, pH 7.4, temperature not specified in the publication	Sus scrofa

IC50 Value

IC50 Value	IC50 Value Maximum	Comment	Organism	Inhibitor	Structure
0.065	-	wild-type, pH 7.4, temperature not specified in the publication	Sus scrofa	3-Mercaptopicolinic acid

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Release 2023.1 (January 2023)